Basic Information
-
Targeting Strategy
-
Gene targeting strategy for B-luc K-562. The stop-CAG-Pr-luciferase-PA-LoxP-PuroR-LoxP sequence was inserted in the hAAVS1 locus of wild-type K-562 cells.
-
Phenotypic Analysis
-
Luminescence signal intensity of B-luc K-562 cells. B-luc K-562 cells displayed strong luminescence signal intensity compared to the control group, as detected by the Bright-GloTM luciferase Assay System (Promega, Cat E2610).
-
In Vivo Imaging Analysis
-
Tumor growth and in vivo imaging of B-luc K-562 cells. B-luc K-562 cells (5×106) were injected by tail vein into B-NDG mice. (A) Imaging was performed on days 0, 4, 7, 11, 21 and 25 for tumor detection and signal intensity analysis. (B) Body weight was measured twice a week (Mean ± SEM). Altogether these data indicate that B-luc K-562 cells can be used for in vivo efficacy evaluation.