Basic Information
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Targeting Strategy
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Gene targeting strategy for B-luc K-562. The stop-CAG-Pr-luciferase-PA-LoxP-PuroR-LoxP sequence was inserted in the hAAVS1 locus of wild-type K-562 cells.
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Phenotypic Analysis
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Luminescence signal intensity of B-luc K-562 cells. B-luc K-562 cells displayed strong luminescence signal intensity compared to the control group, as detected by the Bright-GloTM luciferase Assay System (Promega, Cat E2610).
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In Vivo Imaging Analysis
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Tumor growth and in vivo imaging of B-luc K-562 cells. B-luc K-562 cells (5×106) were injected by tail vein into B-NDG mice. (A) Imaging was performed on days 0, 4, 7, 11, 21 and 25 for tumor detection and signal intensity analysis. (B) Body weight was measured twice a week (Mean ± SEM). Altogether these data indicate that B-luc K-562 cells can be used for in vivo efficacy evaluation.
