Basic Information

Cell Line Name
K562 Gt(AAVS1)tm1(luciferase)/Bcgen
Common Name
B-luc K-562
Related gene
AAVS1adeno-associated virus integration site 1)
Catalog Number
Cell Type/Disease
Leukemia (CML)
Culture Properties
Puromycin, 0.5μg/ml
IMDM+10%FBS+1% L-Glutamine+1% NEAA+1% Sodium pyruvate+1%HEPES
Cryopreservation Medium
90% Complete Medium+10% DMSO
Detecting Instrument
Bright-GloTM luciferase Assay System (Promega, Cat E2610)

Targeting Strategy

Gene targeting strategy for B-luc K-562. The stop-CAG-Pr-luciferase-PA-LoxP-PuroR-LoxP sequence was inserted in the hAAVS1 locus of wild-type K-562 cells.

Phenotypic Analysis

Luminescence signal intensity of B-luc K-562 cells. B-luc K-562 cells displayed strong luminescence signal intensity compared to the control group, as detected by the Bright-GloTM luciferase Assay System (Promega, Cat E2610).

In Vivo Imaging Analysis

Tumor growth and in vivo imaging of B-luc K-562 cells. B-luc K-562 cells (5×106) were injected by tail vein into B-NDG mice. (A) Imaging was performed on days 0, 4, 7, 11, 21 and 25 for tumor detection and signal intensity analysis. (B) Body weight was measured twice a week (Mean ± SEM). Altogether these data indicate that B-luc K-562 cells can be used for in vivo efficacy evaluation.