B-luc K-562

Basic Information

Description

This B-luc K-562 cell line expresses firefly luciferase as a marker of K-562 cells. Luminescence can be observed B-luc K-562 cells.

Targeting Strategy

Gene targeting strategy for B-luc K-562. The luciferase sequence was inserted in the hAAVS1 locus of wild-type K-562.

In vitro luciferase activity of B-luc K-562 cells

Luminescence signal intensity of B-luc K-562 cells. Luminescence intensity was measured using the Bright-Glo^TM luciferase Assay (Promega, Cat E2610). B-luc K-562 cells have a strong luminescence signal. The mix clone and 1-G08 clone of B-luc K-562 cells can be used in vivo experiments.

Quantitative imaging data & body weight changes

Tumor growth and in vivo imaging of B-luc K-562 cells. B-luc K-562 cells (mix, 5×10⁶) were  injected by tail vein into B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed on days 0, 4, 7, 11, 21, and 25, (B) Mice body weight (Mean ± SEM). B-luc K-562 cells can be used for in vivo efficacy evaluation.

Tumor growth and in vivo imaging of B-luc K-562 cells. B-luc K-562 cells (5×10⁶) were injected into the tail vein of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-luc K-562 cells can be used for in vivo efficacy evaluation.