Cell Line Name
MIA PaCa-2 Gt(AAVS1)tm1(luciferase)/Bcgen
B-luc MIA PaCa-2
AAVS1(adeno-associated virus integration site 1)
DMEM+10%FBS+1% L-Glutamine+1% NEAA+1% Sodium pyruvate+1%HEPES
95% Complete Medium+5% DMSO
Bright-GloTM luciferase Assay System (Promega, Cat E2610)
Gene targeting strategy for B-luc MIA PaCa-2. The stop-CAG-Pr-luciferase-PA-LoxP-PuroR-LoxP sequence was inserted in the hAAVS1 locus of wild-type MIA PaCa-2 cells.
In Vivo Imaging Analysis
Tumor growth and in vivo imaging of B-luc MIA PaCa-2 cells. B-luc MIA PaCa-2 cells were subcutaneously inoculated into BALB/c nude mice. Following growth, subcutaneous tumors were excised into small pieces and transplanted into the pancreas of BALB/c nude mice, in situ. (A) Imaging was performed on days 7, 13, 17, 24 and 31 for tumor detection and signal intensity analysis. (B) Body weight was measured twice a week (Mean ± SEM). Altogether these data indicate that B-luc MIA PaCa-2 cells can be used for in vivo efficacy evaluation.