Basic Information
Cell Line Name
MIA PaCa-2 Gt(AAVS1)tm1(luciferase)/Bcgen
Common Name
B-luc MIA PaCa-2
Related gene
AAVS1(adeno-associated virus integration site 1)
Catalog Number
310674
Cell Type/Disease
Pancreas carcinoma
Culture Properties
Adherent
Digestive solution
0.25%Tyrisin
Medium
DMEM+10%FBS+1% L-Glutamine+1% NEAA+1% Sodium pyruvate+1%HEPES
Cryopreservation Medium
95% Complete Medium+5% DMSO
Resistance
Puromycin, 0.5μg/mL
Detecting Instrument
Bright-GloTM luciferase Assay System (Promega, Cat E2610)
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Targeting Strategy
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Gene targeting strategy for B-luc MIA PaCa-2. The stop-CAG-Pr-luciferase-PA-LoxP-PuroR-LoxP sequence was inserted in the hAAVS1 locus of wild-type MIA PaCa-2 cells.
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In Vivo Imaging Analysis
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Tumor growth and in vivo imaging of B-luc MIA PaCa-2 cells. B-luc MIA PaCa-2 cells were subcutaneously inoculated into BALB/c nude mice. Following growth, subcutaneous tumors were excised into small pieces and transplanted into the pancreas of BALB/c nude mice, in situ. (A) Imaging was performed on days 7, 13, 17, 24 and 31 for tumor detection and signal intensity analysis. (B) Body weight was measured twice a week (Mean ± SEM). Altogether these data indicate that B-luc MIA PaCa-2 cells can be used for in vivo efficacy evaluation.
