Basic Information
Description
This B-Luc THP-1 cell line expresses firefly luciferase as a marker of THP-1 cells. Fluorescence could be detected in B-Luc THP-1 cells. B-Luc THP-1 cells can be used to evaluate anti-cancer drugs in acute monocytic leukemia in vivo.
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Gene targeting strategy
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Gene targeting strategy for B-Luc THP-1 cells. The CMV promoter and luciferase were inserted into the targeting vector used in the lentivirus system.
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In vitro luciferase activity
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Luminescence signal intensity of B-Luc THP-1 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc THP-1 cells have a strong luminescence signal. The 1-D09 clone of B-Luc THP-1 cells can be used in vivo experiments.
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Quantitative imaging data & body weight changes
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Tumor growth and in vivo imaging of B-Luc THP-1 cells. B-Luc THP-1 cells (1×106) were injected into the tail vein of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc THP-1 cells can be used for in vivo efficacy evaluation.
