Basic Information
Description
The mouse Siglec15 gene was insert into mouse ROSA26 site. Mouse Siglec15 is highly expressed on the surface of B-mSiglec15 MC38 cells.
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Targeting strategy
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The mouse Siglec15 CDS was insert into mouse ROSA26 site in B-mSiglec15 MC38.
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Protein expression analysis
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The Siglec15 expression analysis in B-mSiglec15 MC38 cells by flow cytometry. Single cells were collected from wild-type MC38 and B-mSiglec15 MC38 cells, and analyzed by flow cytometry with anti-human and mouse SIGLEC15 antibody(SIGLEC15-5G12, in house). Mouse Siglec15 was exclusively detectable in B-mSiglec15 MC38 cells but not wild-type MC38 cells. The 2-C12 clone of B-mSiglec15 MC38 cells was used for in vivo experiments.
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Tumor growth curve & Body weight changes
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Subcutaneous homograft tumor growth of B-mSiglec15 MC38 cells. B-mSiglec15 MC38 cells (5×105) and wild-type MC38 cells (5×105 ) were subcutaneously implanted into the C57BL/6N mice (female, 5-8 week-old, n=5).Tumor size and mice body weight were measured twice a week. (A) Tumor average volume ± SEM, (B) Mice body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively. As shown in panel A, B-mSiglec15 MC38 were able to establish tumor in vivo and can be used for efficacy studies.