The exogenous promoter and mouse Egfr coding sequence were inserted into the mouse genome randomly. Mouse Egfr is highly expressed on the surface of B-Tg(mEgfr) MC38 cells.
Gene targeting strategy for B-Tg(mEgfr) MC38 cells. The exogenous promoter and mouse Egfr coding sequence were inserted into the mouse genome randomly.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-Tg(mEgfr) MC38 cells. B-Tg(mEgfr) MC38 cells (1×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(mEgfr) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Cell Line Inoculation Precautions:
The cell inoculation amount is between 2E5-5E5.