Basic Information

Common name
B-Tg(mMeltf) MC38
Catalog number
321893
Aliases
MTf; Mfi2; CD228
Disease
Colon carcinoma
Organism
Mouse
Strain
C57BL/6
Tissue types
Colon
Tissue
Colon
Application
B-Tg(mMeltf) MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Description

The exogenous promoter and mouse Meltf coding sequence were inserted into the mouse genome randomly. Mouse Meltf is highly expressed on the surface of B-Tg(mMeltf) MC38 cells.

Targeting strategy

Gene targeting strategy for B-Tg(mMeltf) MC38 cells. The exogenous promoter and mouse Meltf coding sequence were inserted into the mouse genome randomly.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-Tg(mMeltf) MC38 cells. B-Tg(mMeltf) MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 10-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(mMeltf) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve of individual mice

B-Tg(mMeltf) MC38 tumor cells growth of individual mice. B-Tg(mMeltf) MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 10-week-old, n=6). As shown in panel, B-Tg(mMeltf) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.