B-Tg(mMet) MC38

Basic Information

Common name
B-Tg(mMet) MC38
Catalog number
321875
Aliases
HGF; HGFR; Par4; c-Met; AI838057
Disease
Colon carcinoma
Organism
Mouse
Strain
C57BL/6
Tissue types
Colon
Tissue
Colon
Application
B-Tg(mMet) MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
NCBI Gene ID

Description

The exogenous promoter and mouse Met coding sequence were inserted into the mouse genome randomly. Mouse Met is highly expressed on the surface of B-Tg(mMet) MC38 cells.

Targeting strategy

Gene targeting strategy for B-Tg(mMet) MC38 cells. The exogenous promoter and mouse Met coding sequence were inserted into the mouse genome randomly.

Protein Expression Analysis

Met expression analysis in B-Tg(mMet) MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-Tg(mMet) MC38 cultures were stained with species-specific anti-Met antibody. Mouse Met was detected on the surface of B-Tg(mMet) MC38 cells but not wild-type MC38 cells. The 1-C09 clone of B-Tg(mMet) MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-Tg(mMet) MC38 cells. B-Tg(mMet) MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(mMet) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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