Basic Information

Common name
B-Tg(mTff1) MC38
Catalog number
322115
Aliases
PS2, Bcei
Disease
Colon carcinoma
Organism
Mouse
Strain
C57BL/6
Tissue types
Colon
Tissue
Colon
Application
B-Tg(mTff1) MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Description

The exogenous promoter and mouse Tff1 coding sequence were inserted into the mouse genome randomly. Mouse Tff1 can be secreted by B-Tg(mTff1) MC38 cells.

Targeting strategy

Gene targeting strategy for B-Tg(mTff1) MC38 cells. The exogenous promoter and mouse Tff1 coding sequence were inserted into the mouse genome randomly.

Protein Expression Analysis

Tff1 expression analysis in B-Tg(mTff1) MC38 cells by ELISA. Mouse Tff1 was detected in the supernatant of B-Tg(mTff1) MC38 cells but not wild-type MC38 cells. The 2-F04 clone of B-Tg(mTff1) MC38 cells was used for in vivo experiments

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-Tg(mTff1) MC38 cells. B-Tg(mTff1) MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(mTff1) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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