Basic Information
Strain Name
C57BL/6-Fcgrttm1/Bcgen
Common Name
B-FcRn KO mice
Background
C57BL/6N
Catalog number
110165
Related Gene
FCGRT(Fc fragment of IgG receptor and transporter)
NCBI Gene ID
-
Targeting strategy
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Gene targeting strategy for B-FcRn KO mice.
The exons of mouse Fcgrt 1-5 were knock out in B-Fcrn KO mice.
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mRNA expression analysis
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Strain specific analysis of Fcgrt gene expression in C57BL/6(+/+) and B-FcRn KO mice by RT-PCR.
Kidney was collected from C57BL/6(+/+) mice and homozygous B-FcRn KO mice. Mouse Fcgrt mRNA was detectable in C57BL/6(+/+) mice but not the homozygous B-FcRn KO mice.
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Protein expression analysis
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Strain specific analysis of FcRn expression in C57BL/6(+/+) and the homozygous B-FcRn KO mice by western blot.
Kidney was collected from C57BL/6(+/+) mice and homozygous B-FcRn KO mice. Mouse FcRn was detectable in C57BL/6(+/+) mice but not the homozygous B-FcRn KO mice.
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FcRn-deficient mice have low serum IgG
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Sera from C57BL/6(+/+) mice and B-FcRn KO mice were analyzed by ELISA .
