Basic Information

Strain Name
C57BL/6-Fcgrttm1/Bcgen
Common Name
B-FcRn KO mice
Background
C57BL/6
Catalog number
110165
Related Gene
FCGRT(Fc fragment of IgG receptor and transporter)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-FcRn KO mice.

The exons  of mouse Fcgrt 1-5 were knock out in B-Fcrn KO mice.

mRNA expression analysis

Strain specific analysis of Fcgrt gene expression in C57BL/6(+/+)  and B-FcRn KO mice by RT-PCR.

Kidney was collected from C57BL/6(+/+) mice and homozygous B-FcRn KO mice. Mouse Fcgrt  mRNA was detectable in C57BL/6(+/+) mice but not the homozygous B-FcRn KO mice.

Protein expression analysis

Strain specific analysis of FcRn expression in C57BL/6(+/+)  and the homozygous B-FcRn KO mice by western blot.

Kidney was collected from C57BL/6(+/+) mice and homozygous B-FcRn KO mice. Mouse FcRn was detectable in C57BL/6(+/+) mice but not the homozygous B-FcRn KO mice.

FcRn-deficient mice have low serum IgG

Sera from C57BL/6(+/+) mice and B-FcRn KO mice were analyzed by ELISA .