Basic Information
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Description
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- The AGT gene encodes a protein (pre-angiotensinogen or angiotensinogen precursor) that is expressed in the liver. This protein is cleaved by renin when blood pressure drops, forming angiotensin I. Angiotensin I is then converted into the active form, angiotensin II, by ACE. This process helps regulate blood pressure and body fluid/electrolyte balance and plays a role in conditions like essential hypertension and preeclampsia.
- The exons 1-5 of mouse Agt gene that encode the whole molecule were replaced by human counterparts in B-hAGT mice. The promoter, 5’UTR and 3’UTR region of the mouse gene are replaced by human counterparts.
- Human AGT mRNA was detectable only in heterozygous B-hAGT mice, but not in wild-type C57BL/6 mice.
- Mouse AGT protein was both detectable in wild-type C57BL/6 mice and heterozygous B-hAGT mice. Human AGT was exclusively detectable in heterozygous B-hAGT mice.
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Targeting strategy
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Gene targeting strategy for B-hAGT mice. The exons 1-5 of mouse Agt gene that encode the whole molecule were replaced by human counterparts in B-hAGT mice. The promoter, 5’UTR and 3’UTR region of the mouse gene are replaced by human counterparts.
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mRNA expression analysis
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Species specific analysis of AGT gene expression in wild-type C57BL/6 mice and heterozygous humanized B-hAGT mice by RT-PCR. Livers were collected from wild-type C57BL/6 mice (+/+) and heterozygous B-hAGT mice (H/+). Human AGT mRNA was detectable only in heterozygous B-hAGT mice, but not in wild-type C57BL/6 mice.
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Protein expression analysis in plasma
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Strain specific AGT expression analysis in wild-type C57BL/6 mice and heterozygous humanized B-hAGT mice by ELISA. Plasma was collected from wild-type C57BL/6 mice (+/+) and heterozygous B-hAGT mice (H/+). Expression level of mouse and human AGT were analyzed by ELISA (Mouse AGT ELISA Kit: Abcam , ab245718; Human AGT ELISA Kit: Abcam, ab287170). Mouse AGT was both detectable in wild-type C57BL/6 mice and heterozygous B-hAGT mice. Human AGT was exclusively detectable in heterozygous B-hAGT mice (n=3). Values are expressed as mean ± SEM.
