Basic Information
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Targeting strategy
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Gene targeting strategy for B-hANPEP mice.
The exons 2~21 of mouse Anpep gene that encode the extracellular domain were replaced by human ANPEP exons 2~21 in B-hANPEP mice.
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Protein expression analysis
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Strain specific ANPEP expression analysis in wild-type C57BL/6 and homozygous B-hANPEP mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hANPEP mice (H/H) and analyzed by flow cytometry with species-specific anti-ANPEP antibody. mANPEP was detectable in DCs of wild-type mice. hANPEP was detectable in DCs of homozygous B-hANPEP mice.
Strain specific ANPEP expression analysis in wild-type C57BL/6 and homozygous B-hANPEP mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hANPEP mice (H/H) and analyzed by flow cytometry with species-specific anti-ANPEP antibody. mANPEP was weakly detectable in monocytes/macrophages of wild-type mice. hANPEP was detectable in monocytes/macrophages of homozygous B-hANPEP mice.
Strain specific ANPEP expression analysis in wild-type C57BL/6 and homozygous B-hANPEP mice by flow cytometry. Blood were collected from wild-type C57BL/6 (+/+) and homozygous B-hANPEP mice (H/H) and analyzed by flow cytometry with species-specific anti-ANPEP antibody. mANPEP was weakly detectable in DCs of wild-type mice. hANPEP was weakly detectable in DCs of homozygous B-hANPEP mice.
Strain specific ANPEP expression analysis in wild-type C57BL/6 and homozygous B-hANPEP mice by flow cytometry. Blood were collected from wild-type C57BL/6 (+/+) and homozygous B-hANPEP mice (H/H) and analyzed by flow cytometry with species-specific anti-ANPEP antibody. mANPEP was weakly detectable in monocytes/macrophages of wild-type mice. hANPEP was detectable in monocytes/macrophages of homozygous B-hANPEP mice. Peritoneal washes were collected from wild-type C57BL/6 (+/+) and homozygous B-hANPEP mice (H/H) and analyzed by flow cytometry with species-specific anti-ANPEP antibody. mANPEP was detectable in macrophages of wild-type mice. hANPEP was detectable in macrophages of homozygous B-hANPEP mice.
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mRNA expression analysis
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Strain specific analysis of ANPEP mRNA expression in wild-type C57BL/6 mice and B-hANPEP mice by RT-PCR. Spleen RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hANPEP mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ANPEP primers. Mouse Anpep mRNA was detectable only in wild-type C57BL/6 mice. Human ANPEP mRNA was detectable only in homozygous B-hANPEP mice but not in wild-type mice.
