Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCD300E mice. The full coding sequences of human CD300E gene that is driven by human CD300E promoter are inserted mouse genomic by BAC transgene(RP11-354A1 via SwaI ).
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Protein expression analysis in monocytes
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CD300E expression analysis in F1 B-hCD300E mice by flow cytometry. Blood cells were collected from wild-type C57BL/6 mice (+/+) and F1 B-hCD300E mice, and analyzed by flow cytometry with anti-CD300E antibody (BD Biosciences, 566315). hCD300E was exclusively detectable in monocytes in 1TG0020-0034 TG mice line.
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Protein expression analysis in macrophages
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CD300E expression analysis in F1 B-hCD300E mice by flow cytometry. Blood cells were collected from wild-type C57BL/6 mice (+/+) and F1 B-hCD300E mice, and analyzed by flow cytometry with anti-CD300E antibody (BD Biosciences, 566315). hCD300E was exclusively detectable in macrophages in 1TG0020-0034 TG mice line.
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Protein expression analysis in neutrophils
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CD300E expression analysis in F1 B-hCD300E mice by flow cytometry. Blood cells were collected from wild-type C57BL/6 mice (+/+) and F1 B-hCD300E mice, and analyzed by flow cytometry with anti-CD300E antibody (BD Biosciences, 566315). hCD300E was not detectable in neutrophils.
