Basic Information

Strain name
C57BL/6-Cd3etm2(CD3E)Cd276tm1(CD276)/Bcgen 
Common Name
B-hCD3E/hB7-H3 mice
Background
C57BL/6
Catalog Number
112617
Aliases
CD3E, CD3epsilon, IMD18, T3E, TCRE, CD3 epsilon subunit of T-cell receptor complex; CD276, 4Ig-B7-H3, B7-H3, B7H3, B7RP-2, CD276 molecule
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCD3E/hB7-H3 mice. 

The exons 2-6 of mouse Cd3e gene that encode the extracellular domain were replaced by human CD3E exons 2-7 in B-hCD3E/hB7-H3 mice

The exon 3-4 of mouse Cd276 gene that encode the extracellular domain were replaced by human CD276 exon 3-6 in B-hCD3E/hB7-H3 mice.

Protein expression analysis

Strain specific CD3E expression analysis in homozygous B-hCD3E/hB7-H3 mice by flow cytometry. 

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hB7-H3 mice (H/H; H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3E/hB7-H3 mice (H/H; H/H) but not in WT mice (+/+).

mRNA and protein expression analysis

Strain specific analysis of B7-H3 expression in wild type (WT) mice and B-hCD3E/hB7-H3 mice by RT-PCR and WB. 

Lung were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hB7-H3 mice (H/H; H/H), and analyzed by RT-PCR and western blot with anti-human B7-H3 antibody. Mouse B7-h3 mRNA was detectable only in lung of WT mice (+/+). Human B7-H3 mRNA was detectable only in homozygous B-hCD3E/hB7-H3 mice (H/H; H/H) but not in WT mice (+/+). The positive band was confirmed to be correct by sequencing. Human B7-H3 was exclusively detectable in homozygous B-hCD3E/hB7-H3 mice (H/H; H/H).

Summary

  • mRNA expression analysis:

Mouse B7-h3 mRNA was detectable only in lung of WT mice (+/+). Human B7-H3 mRNA was detectable only in homozygous B-hCD3E/hB7-H3 mice (H/H; H/H) but not in WT mice (+/+). The positive band was confirmed to be correct by sequencing.

  • Protein expression analysis:

Mouse CD3E was detectable on T cells of WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3E/hB7-H3 mice (H/H; H/H) but not in WT mice (+/+).

Human B7-H3 was exclusively detectable in homozygous B-hCD3E/hB7-H3 mice (H/H; H/H).

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