Basic Information

Strain name
C57BL/6Cd40tm1(CD40)Fcgrttm1(FCGRT)/Bcgen
Common name
B-hCD40/hFcRn mice
Background
C57BL/6N
Catalog number
111912
Aliases
CD40 (Bp50, CDW40, TNFRSF5, p50) FCGRT (FCRN, FcgammaRn, alpha-chain)
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hCD40/hFcRn mice. Exons 2-7 of mouse Cd40 gene that encode the extracellular sequence were replaced by human CD40 exons 2-7 in B-hCD40/hFcRn mice. The human full-length FCGRT cDNA sequence was inserted in the Fcgrt exons 2-4 in B-hCD40/hFcRn mice.

Protein expression analysis

Species-specific CD40 protein expression analysis in wild-type and humanized B-hCD40/hFcRn mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD40/hFcRn (H/H) mice and analyzed by flow cytometry using species-specific anti-CD40 antibodies. Murine CD40 protein was detected in wild-type mice, while human CD40 protein was detected in B-hCD40/hFcRn mice.

Species-specific FcRn protein expression analysis in wild-type and humanized B-hCD40/hFcRn mice. Liver tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD40/hFcRn (H/H) mice and analyzed by western blot using species-specific anti-FcRn antibodies. Murine FcRn protein was detected in wild-type mice, while human FcRn protein was detected in B-hCD40/hFcRn mice.