Basic Information

Strain name
C57BL/6-Cd55tm1(CD55)/Bcgen
Common name
B-hCD55 mice
Background
C57BL/6
Catalog number
111209
Aliases
CR; TC; DAF; CROM; CHAPLE
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCD55 mice. The full coding sequence of mouse Cd55 and Cd55b gene was replaced by human CD55 full coding region gene sequences and 3’UTR in B-hCD55 mice.

  • Mouse Cd55 and Cd55b have 85% identity in the full coding region.

Protein expression analysis

Species-specific CD55 protein expression analysis in humanized B-hCD55 mice by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hCD55 (H/+) mice, and analyzed by flow cytometry using species-specific anti-CD55 antibodies. Murine CD55 protein was detected in T/B cells from wild-type and B-hCD55 mice, while human CD55 protein was exclusively detected in B-hCD55 mice.