CR; TC; DAF; CROM; CHAPLE
NCBI Gene ID
Gene targeting strategy for B-hCD55 mice. The full coding sequence of mouse Cd55 and Cd55b gene was replaced by human CD55 full coding region gene sequences and 3’UTR in B-hCD55 mice.
- Mouse Cd55 and Cd55b have 85% identity in the full coding region.
Protein expression analysis
Species-specific CD55 protein expression analysis in humanized B-hCD55 mice by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hCD55 (H/+) mice, and analyzed by flow cytometry using species-specific anti-CD55 antibodies. Murine CD55 protein was detected in T/B cells from wild-type and B-hCD55 mice, while human CD55 protein was exclusively detected in B-hCD55 mice.