Basic Information
Strain name
C57BL/6-Cd55tm1(CD55)/Bcgen
Common name
B-hCD55 mice
Background
C57BL/6
Catalog number
111209
Aliases
CR; TC; DAF; CROM; CHAPLE
NCBI Gene ID
-
Targeting strategy
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Gene targeting strategy for B-hCD55 mice. The full coding sequence of mouse Cd55 and Cd55b gene was replaced by human CD55 full coding region gene sequences and 3’UTR in B-hCD55 mice.
- Mouse Cd55 and Cd55b have 85% identity in the full coding region.
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Protein expression analysis
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Strain specific CD55 expression analysis in heterozygous B-hCD55 mice by flow cytometry. Splenocytes were collected from wild-type and heterozygous B-hCD55 (H/+) mice, and analyzed by flow cytometry with species-specific anti-CD55 antibody. Mouse CD55 was detectable in T cells and B cells of wild-type C57BL/6 mice and heterozygous B-hCD55 (H/+) mice. Human CD55 was exclusively detectable in heterozygous B-hCD55 mice but not wild-type mice.
