Basic Information

Strain name
C57BL/6-Cd55tm1(CD55)Bcgen/Bcgen
Common name
B-hCD55 mice
Background
C57BL/6
Catalog number
111209
Aliases
CR; TC; DAF; CROM; CHAPLE
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCD55 mice. The full coding sequence of mouse Cd55 and Cd55b gene was replaced by human CD55 full coding region gene sequences and 3’UTR in B-hCD55 mice.

  • Mouse Cd55 and Cd55b have 85% identity in the full coding region.

Protein expression analysis

Strain specific CD55 expression analysis in wild-type C57BL/6 and homozygous B-hCD55 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hCD55 mice (H/H) (n=3, 8-week-old) and analyzed by flow cytometry with species-specific anti-CD55 antibody. mCD55 was detectable in T, B cells of wild-type mice. hCD55 was detectable in T, B cells of homozygous mice.

Strain specific CD55 expression analysis in wild-type C57BL/6 and homozygous B-hCD55 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hCD55 mice (H/H) (n=3, 8-week-old) and analyzed by flow cytometry with species-specific anti-CD55 antibody. mCD55 was detectable in NK and DCs of wild-type mice. hCD55 was detectable in NK and DCs of homozygous mice.

Strain specific CD55 expression analysis in wild-type C57BL/6 and homozygous B-hCD55 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hCD55 mice (H/H) (n=3, 8-week-old) and analyzed by flow cytometry with species-specific anti-CD55 antibody. mCD55 was detectable in monocytes and macrophages of wild-type mice. hCD55 was detectable in monocytes and macrophages of homozygous mice.

Strain specific CD55 expression analysis in wild-type C57BL/6 and homozygous B-hCD55 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hCD55 mice (H/H) (n=3, 8-week-old) and analyzed by flow cytometry with species-specific anti-CD55 antibody. mCD55 was detectable in neutrophils of wild-type mice. hCD55 was detectable in neutrophils of homozygous mice.

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