Basic Information

Strain Name
C57BL/6-Cd9tm1(CD9)/Bcgen
Common Name
B-hCD9 mice
Background
C57BL/6J
Catalog number
111222
Aliases
CD9 (BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29)
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hCD9 mice. Part of exon 2 and exons 3-8 of mouse Cd9 gene sequences were replaced by human CD9 counterpart gene sequences in B-hCD9 mice.

Protein expression analysis

Speciesspecific CD9 protein expression analysis in wild-type and humanized B-hCD9 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD9 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD9 antibodies. Murine CD9 protein was detected in wild-type mice, while human CD9 protein was detected in B-hCD9 mice.

Speciesspecific CD9 protein expression analysis in wild-type and humanized B-hCD9 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD9 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD9 antibodies. Murine CD9 protein was detected in wild-type mice, while human CD9 protein was detected in B-hCD9 mice.

 

Species-specific CD9 protein expression analysis in wild-type and humanized B-hCD9 mice. Bone marrow was isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD9 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD9 antibodies. Murine CD9 protein was detected in wild-type mice, while human CD9 protein was detected in B-hCD9 mice.