Basic Information

Strain Name
C57BL/6-Cd9tm1(CD9)Bcgen/Bcgen
Common Name
B-hCD9 mice
Background
C57BL/6
Catalog number
111222
Aliases
CD9 (BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29)
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hCD9 mice. 

The part of exon 2 and exons 3-8 of mouse Cd9 gene sequences were replaced by human CD9 counterpart gene sequences in B-hCD9 mice.

Protein expression analysis

Detection of hCD9 protein expression in homozygous mice by flow cytometry in granulocytes

Strain specific CD9 expression analysis in homozygous B-hCD9 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD9 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD9 antibody. Mouse CD9 was detectable in wild-type mice. Human CD9 was exclusively detectable in homozygous B-hCD9 but not in wild-type mice.

Detection of hCD9 protein expression in homozygous mice by flow cytometry in macrophages

Strain specific CD9 expression analysis in homozygous B-hCD9 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD9 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD9 antibody. Mouse CD9 was detectable in wild-type mice. Human CD9 was exclusively detectable in homozygous B-hCD9 but not in wild-type mice.

Detection of hCD9 protein expression in homozygous mice by flow cytometry in granulocytes

Strain specific CD9 expression analysis in homozygous B-hCD9 mice by flow cytometry. Bone marrow were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD9 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD9 antibody. Mouse CD9 was detectable in wild-type mice. Human CD9 was exclusively detectable in homozygous B-hCD9 but not in wild-type mice.

Summary

Protein expression analysis:

Human CD9 was detectable in homozygous B-hCD9 mice but not in wild-type mice, and mouse CD9 was detectable only in wild-type mice.

Back to top