Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hCD9 mice. Part of exon 2 and exons 3-8 of mouse Cd9 gene sequences were replaced by human CD9 counterpart gene sequences in B-hCD9 mice.
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Protein expression analysis
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Species–specific CD9 protein expression analysis in wild-type and humanized B-hCD9 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD9 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD9 antibodies. Murine CD9 protein was detected in wild-type mice, while human CD9 protein was detected in B-hCD9 mice.
Species–specific CD9 protein expression analysis in wild-type and humanized B-hCD9 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD9 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD9 antibodies. Murine CD9 protein was detected in wild-type mice, while human CD9 protein was detected in B-hCD9 mice.
Species-specific CD9 protein expression analysis in wild-type and humanized B-hCD9 mice. Bone marrow was isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD9 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD9 antibodies. Murine CD9 protein was detected in wild-type mice, while human CD9 protein was detected in B-hCD9 mice.
