Basic Information
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Description
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- ECSCR, also known as ECSM2 or ARIA, is a type I transmembrane protein. It has been reported that ECSCR can be used as a TAA target for hepatocellular carcinoma. The mechanism may be its effect on the vascular integrity and stability of ECSCR. Interference with ECSCR leads to increased vascular permeability. In addition, other studies have shown that ECSCR regulates endothelial cell signaling. ECSCR is highly expressed in white and brown adipose, and it can regulate energy metabolism and glucose homeostasis by regulating endothelial cell function, and the inactivation of ECSCR enhances insulin sensitivity, making it a potential therapeutic target for diabetes.
- Human ECSCR protein was only detectable in skin and fat of homozygous B-hECSCR mice, but not in wild-type mice. Human ECSCR mRNA was only detectable in lung of homozygous B-hECSCR mice.
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Targeting strategy
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Gene targeting strategy for B-hECSCR mice.
The exons 3-5 of mouse Ecscr gene that encode the extracellular domain were replaced by human ECSCR exons 2-6 in B-hECSCR mice. The genomic region of mouse Ecscr gene that encodes signal peptide and cytoplasmic portion is retained. The promoter, 5’UTR and 3’UTR region of the mouse gene are also retained. The chimeric ECSCR expression is driven by endogenous mouse Ecscr promoter, while mouse Ecscr gene transcription and translation will be disrupted.
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mRNA expression analysis
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Strain specific analysis of ECSCR mRNA expression in wild-type C57BL/6 mice and B-hECSCR mice by RT-PCR. Lung RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hECSCR mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ECSCR primers. Mouse Ecscr mRNA was only detectable in wild-type C57BL/6 mice, while human ECSCR mRNA was only detectable in homozygous B-hECSCR mice.
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Protein expression analysis
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Western blot analysis of ECSCR protein expression in homozygous B-hECSCR mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hECSCR mice (H/H), and then analyzed by western blot with species-specific anti-ECSCR antibody (Santa, sc-515896). 40 μg total proteins were loaded for western blotting analysis. hECSCR was only detectable in skin and fat of homozygous B-hECSCR mice, but not in wild-type mice.
