Basic Information

Strain Name
C57BL/6-Egfrtm2(EGFR)/Bcgen
Common Name
B-hEGFR mice
Background
C57BL/6
Catalog number
120771
Aliases
EGFR, ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA, epidermal growth factor receptor
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hEGFR mice. The exons 2-17 of mouse Egfr gene that encode the extracellular domain were replaced by human EGFR exons 2-17 in B-hEGFR mice.

mRNA expression analysis

Strain specific analysis of EGFR gene expression in wild type (WT) mice and B-hEGFR mice by RT-PCR. Mouse Egfr mRNA was detectable only in liver of WT mice (+/+). Human EGFR mRNA was detectable only in homozygous B-hEGFR mice (H/H) but not in WT mice (+/+).

IHC analysis

Immunohistochemical (IHC) analysis of EGFR expression in homozygous B-hEGFR mice. Liver and kidney tissues were collected from wild-type C57BL/6 (+/+) and homozygous B-hEGFR (H/H) mice and analyzed by IHC using anti-EGFR antibodies. Mouse EGFR was detected in wild-type mice, while human EGFR was detected in B-hEGFR mice. The arrow indicates tissue cells with positive EGFR staining (brown).

Blood routine test

Complete blood count (CBC). Blood from C57BL/6 and B-hEGFR mice (female, 9 week-old,n=8) was collected and analyzed for CBC. There was no differences among any measurement between C57BL/6 and B-hEGFR mice, indicating that introduction of hEGFR in place of its mouse counterpart does not change blood cell composition and morphology. Values are expressed as mean ± SEM.

Blood chemistry test

Blood chemistry tests of B-hEGFR mice. Serum from the C57BL/6 and B-hEGFR mice (female, 9 week-old, n=8) was collected and analyzed for levels of ALT and AST. There was no differences on either measurement between C57BL/6 and B-hEGFR mice, indicating that introduction of hEGFR in place of its mouse counterpart does not change ALT and AST levels or health of liver. Values are expressed as mean ± SEM.