Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hEGFR mice. The exons 2-17 of mouse Egfr gene that encode the extracellular domain were replaced by human EGFR exons 2-17 in B-hEGFR mice.
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mRNA expression analysis
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Strain specific analysis of EGFR gene expression in wild type (WT) mice and B-hEGFR mice by RT-PCR. Mouse Egfr mRNA was detectable only in liver of WT mice (+/+). Human EGFR mRNA was detectable only in homozygous B-hEGFR mice (H/H) but not in WT mice (+/+).
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IHC analysis
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Immunohistochemical (IHC) analysis of EGFR expression in homozygous B-hEGFR mice. Liver and kidney tissues were collected from wild-type C57BL/6 (+/+) and homozygous B-hEGFR (H/H) mice and analyzed by IHC using anti-EGFR antibodies. Mouse EGFR was detected in wild-type mice, while human EGFR was detected in B-hEGFR mice. The arrow indicates tissue cells with positive EGFR staining (brown).
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Blood routine test
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Complete blood count (CBC). Blood from C57BL/6 and B-hEGFR mice (female, 9 week-old,n=8) was collected and analyzed for CBC. There was no differences among any measurement between C57BL/6 and B-hEGFR mice, indicating that introduction of hEGFR in place of its mouse counterpart does not change blood cell composition and morphology. Values are expressed as mean ± SEM.
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Blood chemistry test
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Blood chemistry tests of B-hEGFR mice. Serum from the C57BL/6 and B-hEGFR mice (female, 9 week-old, n=8) was collected and analyzed for levels of ALT and AST. There was no differences on either measurement between C57BL/6 and B-hEGFR mice, indicating that introduction of hEGFR in place of its mouse counterpart does not change ALT and AST levels or health of liver. Values are expressed as mean ± SEM.