Gene targeting strategy for B-hEMP2 mice.
The exons 2-5 of mouse Emp2 gene that encodes the full-length protein was replaced by human EMP2 exons 2-5 in B-hEMP2 mice.
mRNA expression analysis
Species-specific EMP2 gene expression analysis in wild-type and humanized B-hEMP2 mice by RT-PCR. Mouse Emp2 mRNA was detected in lung tissue isolated from wild-type C57BL/6 (+/+) mice, while human EMP2 mRNA was detected in homozygous B-hEMP2 (H/H) mice.