Basic Information

Strain name
C57BL/6NEva1tm1(EVA-1)Bcgen/Bcgen
Common Name
B-hEVA-1 mice
Background
C57BL/6N
Catalog Number
112351
Aliases
EVA-1(DFNB111, EVA, EVA1)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hEVA-1 mice. The exons 2-4 of mouse Eva1 gene that encode the extracellular domain were replaced by human EVA-1 exons 2-4 in B-hEVA-1 mice.

mRNA and protein expression analysis

Strain specific analysis of EVA-1 gene expression in wild-type mice and B-hEVA-1 mice by RT-PCR. Mouse Eva1 mRNA was detectable in thymus of wild-type mice (+/+). Human EVA-1 mRNA was detectable only in homozygous B-hEVA-1 mice(H/H) but not in wild-type mice.

Strain specific EVA-1 expression analysis in homozygous B-hEVA-1 mice by flow cytometry.

Granulocytes, macrophages, dendritic cells from blood were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hEVA-1 mice(H/H), and analyzed by flow cytometry with anti-EVA-1 antibody. Human EVA-1 was detectable in wild-type mice and homozygous B-hEVA-1 mice, as the antibody is crossly reactive with EVA-1 in human and mice.

Protein expression analysis in thymus

Strain specific EVA-1 expression analysis in homozygous B-hEVA-1 mice by flow cytometry.

DN3 cells, epithelial cells from thymus were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hEVA-1 mice(H/H), and analyzed by flow cytometry with anti-EVA-1 antibody. EVA-1 was detectable only in wild-type mice but not in B-hEVA-1 mice .

Summary

mRNA and Protein expression analysis:

Human EVA-1 mRNA was detectable in B-hEVA-1 mice.

Human EVA-1 was detectable on granulocytes, macrophages, dendritic cells from blood in homozygous B-hEVA-1 mice . Human EVA-1 was not detectable on DN3 cells, epithelial cells from thymus in homozygous B-hEVA-1 mice. The results were consistent with the expression profile of human EVA-1.

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