GPC1, glypican, glypican 1
NCBI Gene ID
Gene targeting strategy
Gene targeting strategy for B-hGPC1 mice. Exons 2-9 of mouse Gpc1 gene that encode the full-length protein without signal peptide were replaced by human GPC1 exons 2-9 in B-hGPC1 mice.
mRNA expression analysis
Species-specific GPC1 gene expression analysis in wild-type and humanized B-hGPC1 mice by RT-PCR. Mouse Gpc1 mRNA was detected in lung tissue isolated from wild-type C57BL/6 (+/+) mice, while human GPC1 mRNA was detected in homozygous B-hGPC1 (H/H) mice.
Protein expression analysis
Human GPC1 protein expression analysis in wild-type and humanized B-hGPC1 mice. Cardiac tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hGPC1 (H/H) mice and analyzed by western blot using an anti-human GPC1 antibody. Human GPC1 protein was detected in B-hGPC1 mice.