Basic Information
Strain name
C57BL/6N-Gpc1tm1(GPC1)Bcgen/Bcgen
Common Name
B-hGPC1 mice
Background
C57BL/6
Catalog Number
111239
Aliases
GPC1, glypican, glypican 1
NCBI Gene ID
-
Gene targeting strategy
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Gene targeting strategy for B-hGPC1 mice. Exons 2-9 of mouse Gpc1 gene that encode the full-length protein without signal peptide were replaced by human GPC1 exons 2-9 in B-hGPC1 mice.
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mRNA expression analysis
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Strain specific analysis of GPC1 gene expression in wild-type mice and B-hGPC1 mice by RT-PCR. Mouse Gpc1 mRNA was detectable only in lung of wild-type mice (+/+). Human GPC1 mRNA was detectable only in homozygous B-hGPC1 mice (H/H) but not in wild-type mice (+/+).
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Protein expression analysis
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Strain specific GPC1 expression analysis in homozygous B-hGPC1 mice by western blot. Heart was collected from wild-type mice (+/+) and homozygous B-hGPC1 mice (H/H), and analyzed by western blot with anti-GPC1 antibody. Human GPC1 was detectable in homozygous B-hGPC1 mice (H/H).
