Basic Information

Strain Name
C57BL/6-Erbb2tm1(ERBB2)Tacstd2tm1(TACSTD2)/Bcgen
Common Name
B-hHER2/hTROP2 mice
Background
C57BL/6
Catalog number
111881
Related Genes
TTACSTD2, EGP-1, EGP1, GA733-1, GA7331, GP50, M1S1, TROP2, tumor associated calcium signal transducer 2,  Trophoblast cell-surface antigen 2 ; ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, VSCN2
NCBI Gene ID

mRNA expression analysis

Species-specific HER2 and TROP2 gene expression analyses in wild-type and humanized B-hHER2/hTROP2 mice by RT-PCR. Mouse Her2 and Trop2 mRNAs were detected in kidney tissue isolated from wild-type C57BL/6 (+/+) mice, while human HER2 and TROP2 mRNAs were detected in homozygous B-hHER2/hTROP2 (H/H) mice.

Protein expression analysis

Species-specific TROP2 protein expression analysis in wild-type and humanized B-hHER2/hTROP2 mice by western blot. Kidney tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hHER2/hTROP2 (H/H) mice, and analyzed by western blot using an anti-TROP2 antibody. TROP2 protein was detected in wild-type and B-hHER2/hTROP2 mice due to the cross-reactive antibody.

IHC analysis of HER2 expression

Immunohistochemical (IHC) analysis of HER2 expression in wild-type and humanized B-hHER2/hTROP2 mice. Liver tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hHER2/hTROP2 (H/H) mice and analyzed by IHC using an anti-HER2 antibody. HER2 protein was detected in wild-type and B-hHER2/hTROP2 mice due to the cross-reactive antibody. The arrow indicates tissue cells with positive HER2 staining (brown).