Basic Information

Strain Name
C57BL/6-Itga4tm1(ITGA4)Bcgen/Bcgen
Common Name
B-hITGA4 mice
Background
C57BL/6
Catalog Number
110902
Related Gene
integrin subunit alpha 4, CD49D, IA4
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hITGA4 mice. The exons 2-27 of mouse Itga4 gene that encode the extracellular domain were replaced by human ITGA4 exons 2-27 in B-hITGA4 mice.

mRNA expression analysis

Strain specific analysis of ITGA4 gene expression in wild-type mice and homozygous B-hITGA4 mice by RT-PCR. Mouse Itga4 mRNA was detectable in splenocytes of wild-type mice(+/+). Human ITGA4 mRNA was detectable in homozygous B-hITGA4 mice(H/H) .

Protein expression analysis

DCs

Strain specific ITGA4 expression analysis in homozygous B-hITGA4 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice(+/+) and homozygous B-hITGA4 mice(H/H). Mouse ITGA4 was exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 was exclusively detectable in homozygous B-hITGA4 mice.

NK cells

Strain specific ITGA4 expression analysis in homozygous B-hITGA4 mice by flow cytometry.  Splenocytes were collected from wild-type C57BL/6 mice(+/+) and homozygous B-hITGA4 mice(H/H). Mouse ITGA4 was exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 was exclusively detectable in homozygous B-hITGA4 mice.

Monocytes

Strain specific ITGA4 expression analysis in homozygous B-hITGA4 mice by flow cytometry.  Splenocytes were collected from wild-type C57BL/6 mice(+/+) and homozygous B-hITGA4 mice(H/H). Mouse ITGA4 was exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 was exclusively detectable in homozygous B-hITGA4 mice.

Macrophages

Strain specific ITGA4 expression analysis in homozygous B-hITGA4 mice by flow cytometry.  Splenocytes were collected from wild-type C57BL/6 mice(+/+) and homozygous B-hITGA4 mice(H/H). Mouse ITGA4 was exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 was exclusively detectable in homozygous B-hITGA4 mice.

Summary

mRNA expression analysis

Mouse Itga4 mRNA was detectable in splenocytes of wild-type mice(+/+). Human ITGA4 mRNA was detectable in homozygous B-hITGA4 mice(H/H).

Protein expression analysis

Mouse ITGA4 was exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 was exclusively detectable in homozygous B-hITGA4 mice.

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