Basic Information

Strain Name
C57BL/6-Itga4tm1(ITGA4)/Bcgen
Common Name
B-hITGA4 mice
Background
C57BL/6J
Catalog Number
110902
Related Gene
integrin subunit alpha 4, CD49D, IA4
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hITGA4 mice. The exons 2-27 of mouse Itga4 gene that encode the extracellular domain were replaced by human ITGA4 exons 2-27 in B-hITGA4 mice.

Protein expression analysis

Species-specific ITGA4 protein expression analysis in wild-type and humanized B-hITGA4 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hITGA4 (H/+) mice, and analyzed by flow cytometry using species-specific anti-ITGA4 antibodies. Murine ITGA4 protein was detected in wild-type and B-hITGA4 mice, while human ITGA4 protein was detected in B-hITGA4 mice.