Gene targeting strategy for B-hITGB1 mice.
The exons 2-15 of mouse Itgb1 gene were replaced by human ITGB1 exons 2-15 in B-hITGB1 mice.
Protein expression analysis
Strain specific ITGB1 expression analysis in heterozygous B-hITGB1 mice by flow cytometry.
Splenocytes were collected from C57BL/6 wild-type mice (+/+) and heterozygous B-hITGB1 mice (H/+) analyzed by flow cytometry with species-specific anti-ITGB1 antibody. Mouse ITGB1 was detectable in wild-type mice and heterozygous B-hITGB1 mice (H/+). Human ITGB1 were exclusively detectable in heterozygous B-hITGB1.