Basic Information

Strain name
C57BL/6-Jag1tm1(JAG1)Bcgen/Bcgen
Common Name
B-hJAG1 mice
Background
C57BL/6
Catalog Number
110997
Aliases
AGS, AHD, AWS, HJ1, AGS1, DCHE, CD339, JAGL1, CMT2HH
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hJAG1 mice. The exons 2~5 of mouse Jag1 gene that encode the extracellular domain were replaced by human JAG1 exons 2~5 in B-hJAG1 mice.

 

Protein expression analysis

Western blot analysis of JAG1 protein expression in homozygous B-hJAG1 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hJAG1 mice (H/H), and then analyzed by western blot with anti-JAG1 antibody. 72 μg total proteins were loaded for western blotting analysis. JAG1 protein was detected in the kidney, lung, heart, spleen of wild-type C57BL/6 mice and homozygous B-hJAG1 mice, as the antibody is cross-recognize both human and mouse JAG1.

mRNA expression analysis

Strain specific analysis of JAG1 mRNA expression in wild-type C57BL/6 mice and B-hJAG1 mice by RT-PCR. Lung RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hJAG1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human JAG1 primers. Mouse Jag1 mRNA was detected in wild-type C57BL/6 mice. Human JAG1 mRNA was detected in homozygous B-hJAG1 mice but not in wild-type mice.

Summary

mRNA expression analysis:

Mouse Jag1 mRNA was detected in lung of wild-type C57BL/6 mice. Human JAG1 mRNA was detected in lung of B-hJAG1 mice.

Protein expression analysis:

JAG1 protein was detected in the kidney, lung, heart, spleen of wild-type C57BL/6 mice and homozygous B-hJAG1 mice, as the antibody is cross-recognize both human and mouse JAG1.

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