Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hL1CAM mice.
The exons 2-26 of mouse L1cam gene that encode the extracellular domain and the transmembrane domain were replaced by human L1CAM exons 2-26 in B-hL1CAM mice.
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Protein expression analysis
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Strain specific L1CAM expression analysis in homozygous B-hL1CAM mice by flow cytometry.
Brain was collected from wild type (WT) mice (+/+) and homozygous B-hL1CAM mice (H/H), and analyzed by flow cytometry with species-specific anti-L1CAM antibody. Mouse L1CAM was detectable in WT mice (+/+). Human L1CAM was exclusively detectable in homozygous B-hL1CAM mice (H/H) but not in WT mice (+/+).
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Summary
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Protein expression analysis:
Human L1CAM was exclusively detectable in brain of homozygous B-hL1CAM mice (H/H) but not in WT mice (+/+), and mouse L1CAM was detectable only in WT mice (+/+).
