Basic Information

Strain Name
C57BL/6-Slc7a5tm1(SLC7A5)/Bcgen
Common Name
B-hLAT1 mice
Background
C57BL/6J
Catalog number
110998
Aliases
SLC7A5, 4F2LC, CD98, D16S469E, E16, LAT1,  MPE16, solute carrier family 7 member 5 [Homo sapiens
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hLAT1 mice.

Exons 1-10 of the mouse Slc7a5 gene (encoding the full-length protein) were replaced by human SLC7A5 exons 1-10 to generate humanized B-hLAT1 mice.

mRNA expression analysis

Strain specific analysis of LAT1 gene expression in wild type (WT) mice and B-hLAT1 mice by RT-PCR. Mouse Slc7a5 mRNA was detectable only in brain of WT mice (+/+). Human SLC7A5 mRNA was detectable in homozygous B-hLAT1 mice (H/H) but not in WT mice (+/+).

Protein expression analysis

Strain specific LAT1 expression analysis in homozygous B-hLAT1 mice by western blot. Brain was collected from wild type (WT) mice (+/+) and homozygous B-hLAT1 mice (H/H), and analyzed by western blot with anti-LAT1 antibody. LAT1 was detectable in WT mice (+/+) and homozygous B-hLAT1 mice (H/H) due to the cross-reactivity of antibodies. Human LAT1 was exclusively detectable in homozygous B-hLAT1 mice (H/H).