Basic Information

Strain name
C57BL/6NLgals3bptm1(LGALS3BP)/Bcgen
Common Name
B-hLGALS3BP mice
Background
C57BL/6N
Catalog Number
112343
Aliases
LGALS3BP(90K, BTBD17B, CyCAP, M2BP, MAC-2-BP, TANGO10B, gp90)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hLGALS3BP mice.

The exons 2-6 of mouse Lgals3bp gene that  encode the full-length protein were replaced by human LGALS3BP exons 2-6 in B-hLGALS3BP mice.

mRNA and protein expression analysis

Strain specific analysis of LGALS3BP gene expression in wild-type mice and B-hLGALS3BP mice by RT-PCR. Mouse Lgals3bp mRNA was detectable in small intestine of wild-type mice (+/+). Human LGALS3BP mRNA was detectable only in homozygous B-hLGALS3BP mice(H/H) but not in wild-type C57BL/6 mice.

Strain specific LGALS3BP protein expression analysis in homozygous B-hLGALS3BP mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hLGALS3BP mice (H/H), and analyzed by ELISA with species-specific LGALS3BP ELISA kit. Human LGALS3BP was exclusively detectable in homozygous B-hLGALS3BP mice but not in wild-type mice.

Summary

mRNA expression analysis:

Mouse Lgals3bp mRNA was detectable in small intestine of wild-type (+/+) mice. Human LGALS3BP mRNA was detectable in B-hLGALS3BP mice(H/H) but not in wild-type mice.

Protein expression analysis:

Human LGALS3BP was exclusively detectable in homozygous B-hLGALS3BP mice but not in wild-type mice.

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