Basic Information
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Targeting strategy
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Gene targeting strategy for B-hLGALS3BP mice. The exons 2-6 of mouse Lgals3bp gene that encode the full-length protein were replaced by human LGALS3BP exons 2-6 in B-hLGALS3BP mice.
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mRNA and protein expression analysis
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Strain specific analysis of LGALS3BP gene expression in wild-type mice and B-hLGALS3BP mice by RT-PCR. Mouse Lgals3bp mRNA was detectable in small intestine of wild-type mice (+/+). Human LGALS3BP mRNA was detectable only in homozygous B-hLGALS3BP mice(H/H) but not in wild-type C57BL/6 mice.
Strain specific LGALS3BP protein expression analysis in homozygous B-hLGALS3BP mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hLGALS3BP mice (H/H), and analyzed by ELISA with species-specific LGALS3BP ELISA kit. Human LGALS3BP was exclusively detectable in homozygous B-hLGALS3BP mice but not in wild-type mice.
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Summary
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mRNA expression analysis:
Mouse Lgals3bp mRNA was detectable in small intestine of wild-type (+/+) mice. Human LGALS3BP mRNA was detectable in B-hLGALS3BP mice(H/H) but not in wild-type mice.
Protein expression analysis:
Human LGALS3BP was exclusively detectable in homozygous B-hLGALS3BP mice but not in wild-type mice.