Basic Information

Strain name
C57BL/6-Mfge8tm1(MFGE8)Bcgen/Bcgen
Common Name
B-hMFGE8 mice
Background
C57BL/6
Catalog Number
111198
Aliases
MFGE8: BA46, HMFG, MFGM, SED1, Hp47,  EDIL1, MFG-E8, SPAG10, OAcGD3S, HsT19888
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hMFGE8 mice. The exons 1~8 of mouse Mfge8 gene that encodes the full-length protein was replaced by human MFGE8 exons 1~8 in B-hMFGE8 mice.

Protein expression analysis

Strain specific MFGE8 expression analysis in homozygous B-hMFGE8 mice by ELISA. Serum were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hMFGE8 mice (H/H), and then analyzed by ELISA with species-specific MFGE8 ELISA kit. Mouse MFGE8 was only detectable in wild-type mice (A). Human MFGE8 was exclusively detectable in homozygous B-hMFGE8 mice but not in wild-type mice (B).

Summary

Protein expression analysis:

Human MFGE8 was only detectable in homozygous B-hMFGE8 mice, and mouse MFGE8 was detectable only in wild-type C57BL/6 mice.

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