Basic Information
Strain name
C57BL/6-Mfge8tm1(MFGE8)/Bcgen
Common Name
B-hMFGE8 mice
Background
C57BL/6
Catalog Number
111198
Aliases
MFGE8: BA46, HMFG, MFGM, SED1, Hp47,
EDIL1, MFG-E8, SPAG10, OAcGD3S, HsT19888
NCBI Gene ID
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Targeting strategy
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Gene targeting strategy for B-hMFGE8 mice.
The exons 1~8 of mouse Mfge8 gene that encodes the full-length protein was replaced by human MFGE8 exons 1~8 in B-hMFGE8 mice.
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Protein expression analysis
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Strain specific MFGE8 expression analysis in homozygous B-hMFGE8 mice by ELISA. Serum were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hMFGE8 mice (H/H), and then analyzed by ELISA with species-specific MFGE8 ELISA kit. Mouse MFGE8 was only detectable in wild-type mice (A). Human MFGE8 was exclusively detectable in homozygous B-hMFGE8 mice but not in wild-type mice (B).
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Summary
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Protein expression analysis:
Human MFGE8 was only detectable in homozygous B-hMFGE8 mice, and mouse MFGE8 was detectable only in wild-type C57BL/6 mice.
