NCBI Gene ID
Gene targeting strategy for B-hMSLN mice. The mouse Msln gene sequences that encode the full length of coding region were replaced by human MSLN coding gene sequence in B-hMSLN mice.
Protein expression analysis
Strain specific MSLN protein expression analysis in wild-type mice and B-hMSLN mice by western blotting. Mouse MSLN was detectable in heterozygous B-hMSLN mice (H/+) and wild-type mice (+/+). Human MSLN was detectable only in B-hMSLN mice (H/+).
Protein expression analysis:
Human MSLN was detectable only in B-hMSLN mice (H/+).