Basic Information
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Targeting strategy
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Gene targeting strategy for B-hP2RX3 mice.
The exon 2~8 of mouse P2rx3 gene that encodes the full-length protein was replaced by human P2RX3 exon 2~8 in B-hP2RX3 mice.
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Protein expression analysis
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Western blot analysis of P2RX3 protein expression in homozygous B-hP2RX3 mice. Dorsal root ganglia were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hP2RX3 mice (H/H), and then analyzed by western blot with anti-P2RX3 antibody. 30 μg total proteins were loaded for western blotting analysis. P2RX3 was detected in Dorsal root ganglia. Anti-P2RX3 antibody was cross reactive between human and mouse.
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mRNA expression analysis
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Strain specific analysis of P2RX3 mRNA expression in wild-type C57BL/6 mice and B-hP2RX3 mice by RT-PCR. Dorsal root ganglia RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hP2RX3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human P2RX3 primers. Mouse P2rx3 mRNA was detectable only in wild-type C57BL/6 mice. Human P2RX3 mRNA was detectable only in homozygous B-hP2RX3 mice but not in wild-type mice.
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Summary
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mRNA expression analysis:
Mouse P2rx3 mRNA was detectable in the dorsal root ganglia of wild-type C57BL/6 mice. Human P2RX3 mRNA was detectable in B-hP2RX3 mice.
Protein expression analysis:
Human P2RX3 was detectable in the dorsal root ganglia of homozygous B-hP2RX3 mice, and mouse P2rx3 was detectable in wild-type C57BL/6 mice.
