Basic Information
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Targeting strategy
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Gene targeting strategy for B-hPLAT mice. The exons 2-14 of mouse Plat gene that encode the whole molecule are replaced by human counterparts in B-hPLAT mice. The promoter and 5’UTR region of the mouse gene are retained, but the 3’UTR region of the mouse gene are replaced by human counterparts.
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Protein expression analysisProtein expression analysis in serum
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Strain specific PLAT expression analysis in wild-type C57BL/6JNifdc mice and homozygous B-hPLAT mice by ELISA. Serum was collected from female wild-type C57BL/6 mice (+/+) (n=3, 5-week-old) and homozygous B-hPLAT mice (H/H) (n=3, 5-week-old). Expression level of mouse and human PLAT were analyzed by ELISA (anti-mouse PLAT ELISA kit: Abcam, ab233615; anti-human PLAT ELISA kit: Abcam, ab282306). Mouse PLAT was only detectable in wild-type C57BL/6 mice. Human PLAT was exclusively detectable in homozygous B-hPLAT mice (n=3). Values are expressed as mean ± SEM.
