NCBI Gene ID
Gene targeting strategy. Part of exon 2 and exons 3-7 of the mouse Plaur gene was replaced by human PLAUR counterpart gene sequences to generate humanized B-hPLAUR mice.
Protein expression analysis
PLAUR expression analysis in homozygous B-hPLAUR mice by flow cytometry. Peritoneal macrophages were collected from wild-type mice (+/+) and homozygous B-hPLAUR mice (H/H) stimulated with LPS, and analyzed by flow cytometry with species-specific anti-PLAUR antibody. Mouse PLAUR was detectable in wild-type mice. Human PLAUR was detectable in homozygous B-hPLAUR mice.