Basic Information
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Targeting strategy
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Gene targeting strategy for B-hPSCA mice. The full coding region of mouse Psca gene was replaced by human PSCA counterpart gene sequences in B-hPSCA mice.
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mRNA expression analysis
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Strain specific analysis of PSCA gene expression in wild-type mice and B-hPSCA mice by RT-PCR. Mouse Psca mRNA was detectable only in stomach of wild-type mice (+/+). Human PSCA mRNA was detectable only in homozygous B-hPSCA mice (H/H) but not in wild-type C57BL/6 mice.
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Protein expression analysis
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PSCA protein expression analysis in wild-type mice and B-hPSCA mice by western blotting. PSCA was both detectable in B-hPSCA mice and wild-type mice because the antibody has cross reaction between human and mouse. But, it has been confirmed the PSCA sequence was humanization successfully.
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Summary
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mRNA expression analysis:
Mouse Psca mRNA was detectable in the stomach of wild-type (+/+) mice. Human PSCA mRNA was detectable in B-hPSCA mice (H/H) but not in wild-type mice.
Protein expression analysis:
PSCA was both detectable in B-hPSCA mice and wild-type mice because the antibody has cross reaction between human and mouse.
