Basic Information
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Targeting strategy
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Gene targeting strategy for B-hRGMA mice.
The exon 3 and exon 4 of mouse Rgma gene was replaced by human RGMA exon 3 and exon 4 in B-hRGMA mice.
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mRNA expression analysis
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Strain specific analysis of RGMA gene expression in wild-type C57BL/6 mice and hRGMA mice by RT-PCR. Mouse Rgma mRNA was detectable only in the wild-type C57BL/6 mice (+/+). Human RGMA mRNA was detectable only in homozygous B-hRGMA mice (H/H), but not in wild-type mice.
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Protein expression analysis
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Strain specific RGMA expression analysis in homozygous B-hRGMA mice by western blot. Brain tissue was collected from the wild-type mice (+/+) and homozygous B-hRGMA (H/H) mice, and analyzed by western blot with anti-RGMA antibody. RGMA was detectable in wild-type mice (+/+) and homozygous B-hRGMA mice (H/H) due to the cross-reactivity of antibodies.
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Summary
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- mRNA expression analysis:
Mouse Rgma mRNA was detectable in the brain of wild-type (+/+). Human RGMA mRNA was detectable in B-hRGMA mice (H/H) mice but not in wild-type mice.
- Protein expression analysis:
RGMA was detectable in wild-type mice (+/+) and homozygous B-hRGMA mice (H/H) due to the cross-reactivity of antibodies.
