NCBI Gene ID
mRNA expression analysis
Species-specific RGMA gene expression analysis in wild-type and humanized B-hRGMA mice by RT-PCR. Murine Rgma mRNA was detected in wild-type C57BL/6 (+/+) mice, while human RGMA mRNA was exclusively detected in homozygous B-hRGMA (H/H) mice.
Protein expression analysis
Species-specific RGMA protein expression analysis in humanized B-hRGMA mice. Brain tissue was isolated from wild-type (+/+) and homozygous B-hRGMA (H/H) mice, and analyzed by western blot using an anti-m/hRGMA antibody. RGMA protein was detected in wild-type and B-hRGMA mice due to the cross-reactivity of antibodies.