Basic Information
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Description
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- Heat Shock Protein 47 (HSP47), also known as Serpin-H1, is localized to endoplasmic reticulum (ER), where it is a collagen-specific molecular chaperone.
- In the ER, HSP47 interacts with and stabilizes correctly-folded procollagen, which is up-regulated in several cancers including squamous cell carcinoma, breast and prostate carcinomas.
- The exons 1-5 of mouse Serpinh1 gene that encode the whole molecule (ATG to STOP codon), including promoter, 5’UTR and 3’UTR were replaced by human counterparts in B-hSERPINH1 mice. The human SERPINH1 expression is driven by human Serpinh1 promoter, while mouse Serpinh1 gene transcription and translation will be disrupted.
- SERPINH1 protein was detectable in in heart, liver(weak expression), lung(weak expression), kidney(weak expression), uterus and ovary of wild-type C57BL/6JNifdc mice and homozygous B-hSERPINH1 mice, as the antibody can cross-recognize both human and mouse SERPINH1.
- Mouse Serpinh1 mRNA was detectable in wild-type C57BL/6JNifdc mice. Human SERPINH1 mRNA was detectable in homozygous B-hSERPINH1 mice.
- Application: This product is used for pharmacodynamics and safety evaluation of drugs.
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Targeting strategy
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Gene targeting strategy for B-hSERPINH1 mice. The exons 1-5 of mouse Serpinh1 gene that encode the whole molecule (ATG to STOP codon), including promoter, 5’UTR and 3’UTR were replaced by human counterparts in B-hSERPINH1 mice. The human SERPINH1 expression is driven by human SERPINH1 promoter, while mouse Serpinh1 gene transcription and translation will be disrupted.
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mRNA expression analysis in humanized B-hSERPINH1 mice
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Species specific analysis of SERPINH1 gene expression in wild-type C57BL/6JNifdc mice and homozygous humanized B-hSERPINH1 mice by RT-PCR. Lung was collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hSERPINH1 mice (H/H). Mouse Serpinh1 mRNA was detectable in wild-type C57BL/6JNifdc mice. Human SERPINH1 mRNA was detectable only in homozygous B-hSERPINH1 mice, but not in wild-type C57BL/6JNifdc mice.
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Protein expression analysis
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Strain specific SERPINH1 expression analysis in homozygous B-hSERPNH1 mice by western blot. Heart, liver, lung, kidney, uterus and ovary were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hSERPINH1 mice (H/H), and analyzed by western blot with anti-SERPINH1 antibody (proteintech, 10875-1-AP). m/hSERPINH1 was detectable in heart, liver(weak expression), lung(weak expression), kidney(weak expression), uterus and ovary of wild-type C57BL/6JNifdc mice and homozygous B-hSERPINH1 mice.
