Basic Information

Strain name
C57BL/6N-Tlr3tm1(TLR3)Bcgen/Bcgen
Common Name
B-hTLR3 mice
Background
C57BL/6N
Catalog Number
111431
Aliases
CD283, IIAE2, IMD83
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hTLR3 mice. The exons 4~6 of mouse Tlr3 gene that encode the extracellular domain were replaced by human TLR3 exons 2~4 in B-hTLR3 mice.

Protein expression analysis

Strain specific TLR3 expression analysis in homozygous B-hTLR3 mice by flow cytometry. Peritoneal washes and Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTLR3 mice (H/H). Flow cytometry analysis of the Peritoneal washes-macrophages, Splenocytes-macrophages, DCs were analyzed with anti-TLR3 antibody. Mouse TLR3 was detected in Peritoneal washes-macrophages, Splenocytes-macrophages, DCs of wild-type mice. Human TLR3 was detected in Peritoneal washes-macrophages, Splenocytes-macrophages, DCs of homozygous B-hTLR3 mice.

Strain specific TLR3 expression analysis in homozygous B-hTLR3 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTLR3 mice (H/H). Flow cytometry analysis of the monocytes, granulocytes, NK cells were analyzed with anti-TLR3 antibody. Mouse TLR3 was detected in monocytes, granulocytes, NK cells of wild-type mice. Human TLR3 was detected in monocytes, NK cells of homozygous B-hTLR3 mice, but not in granulocytes.

Strain specific TLR3 expression analysis in homozygous B-hTLR3 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTLR3 mice (H/H). Flow cytometry analysis of the T cells and B cells were analyzed with anti-TLR3 antibody. Mouse TLR3 was detected in B cells of wild-type mice. Human TLR3 was detected in B cells of homozygous B-hTLR3 mice.

Summary

Protein expression analysis:

mouse TLR3 was detectable in WT mice. Human TLR3 was detected in homozygous B-hTLR3 mice.

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