Basic Information
Strain Name
C57BL/6JNifdc-Ptprctm1(K302E)Bcgen/Bcgen
Stock Number
112593
Common Name
B-CD45.1 mice
Background
C57BL/6JNifdc
Aliases
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; IMD105
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Targeting strategy
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Gene targeting strategy for B-CD45.1 mice. Introduce a lysine to glutamic acid mutation (K302E) in mouse Ptprc gene, resulting the encoding protein can be recognized by anti-CD45.1 antibodies.
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Thy1.1 expression analysis in B-Thy1.1 mice
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CD45.1 expression analysis in homozygous B-CD45.1 mice by flow cytometry. Spleen, blood and bone marrow were collected from wild-type C57BL/6 mice (+/+) and homozygous B-CD45.1 mice (Mut/Mut), and then analyzed by flow cytometry with anti-CD45.1 or CD45.2 antibodies. CD45.2 was detection in C57BL/6 mice. CD45.1 were only detectable in B-CD45.1 mice but not in wild-type mice.
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Publication