A Novel Mechanism of Spine Damages in Stroke via DAPK1 and Tau
Synaptic spine loss is one of the major preceding consequences of stroke damages, but its underlying molecular mechanisms remain unknown. Here, we report that a direct interaction of DAPK1 with Tau causes spine loss and subsequently neuronal death in a mouse model with stroke. We found that DAPK1 phosphorylates Tau protein at Ser262 (pS(262)) in cortical neurons of stroke mice. Either genetic deletion of DAPK1 kinase domain (KD) in mice (DAPK1-KD(-/-)) or blocking DAPK1-Tau interaction by systematic application of a membrane permeable peptide protects spine damages and improves neurological functions against stroke insults. Thus, disruption of DAPK1-Tau interaction is a promising strategy in clinical management of stroke.
Authors: Pei L1, Wang S2, Jin H2, Bi L2, Wei N2, Yan H2, Yang X2, Yao C2, Xu M2, Shu S2, Guo Y2, Yan H2, Wu J2, Li H2, Pang P2, Tian T2, Tian Q3, Zhu LQ3, Shang Y4, Lu Y5.
Influence Factor: 6.559
Citation: Cereb Cortex 25, 4559-4571 (2015).