Development and characterization of an HPV18 detection kit using two novel HPV18 type-specific monoclonal antibodies

Development and characterization of an HPV18 detection kit using two novel HPV18 type-specific monoclonal antibodies

Abstract

Background

HPV 18 is one of the most prevalent oncogenic types, only second to HPV 16, and included in the licensed vaccines on the market. In this study, we describe the production and characterization of a panel of monoclonal antibodies (mAb) to HPV18.

Methods

The immunocompetence of 1B1 and 4C2 mAbs for HPV L1 protein was evaluated by SDS-PAGE analysis, neutralization assays, affinity identification, and ELISA. The 1B1 and 4C2 genes were sequenced and analyzed. Finally, the detection kit with the two mAbs was assessed for linearity, repeatability and specificity.

Results

Both mAbs specifically recognized HPV18 L1 and virus-like particles (VLPs). These mAbs are conformation-neutralizing antibodies that have high affinity and type specificity. Based on these characteristics of these mAbs, we developed an ELISA kit for specifically detecting HPV 18 antigen. We showed that this kit displayed good linearity, repeatability and sensitivity for detecting HPV18 L1 pentamer and HPV18 VLP.

Conclusions

We characterized two monoclonal neutralizing antibodies for HPV L1 protein, and developed an ELISA kit for specifically detecting HPV 18 antigen. This newly developed kit can be used to monitor the potency of HPV vaccines throughout the entire production process as well as preliminary analysis of HPV18 infections.

Authors: Yao Zhang†, Ye He†, Ling Li†, Shutian Liang, Mei Yan, Dongyan Ren, Zengmin Yang, Wenli Zhao, Luyan Miao, Haijiang Zhang and Yongjiang Liu

Influence Factor: 2.39

Citation: Diagn Pathol 13, 55 (2018).

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