Animal Microbiology
Quarterly Testing Sheet for B-NDG Immunodeficienct Mice |
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ECT | Mycoplasma spp. | Group G beta-hemolytic Streptococcus |
MHV | Corynebacterium kutscheri | In-vitro parasites (arthropods, such as fleas, mites, and lice) |
SV | Pasteurella pneumotropica | Enteric Helminths |
PVM | Klebsiella pneumoniae | Flagellates |
REO-3 | Staphylococcus aureus | Ciliates |
MMV/MVM | Pseudomonas aeruginosa | Toxoplasma gondii |
LCMV | Salmonella group | Pneumocystis carinii |
HV | Yersinia pseudotuberculosis* | Encephalitozoon cuniculi |
TMEV | Yersinia enterocolitica* | Amoeba limicola |
MAV/MAD1, 2 | Streptobacillus moniliformis* | Proteus mirabilis |
POLY | Pathogenic dermal fungi* | |
MPV | Escherichia coli O115 a,C,K(B)* | |
EDIM | Streptococcus pneumoniae* | |
MNV | Beta-hemolytic streptococcus* | |
MCMV | Klebsiella oxytoca* | |
KVIrus | Corynebacterium bovis | |
Parvovirus NS-1 | Helicobacter | |
Lactate dehydrogenase-elevating virus | CARB | |
Clostridium piliforme | Group B beta-hemolytic Streptococcus |
Quarterly Testing Sheet for Immune Checkpoint Humanized Mice |
|
No. | Test Item |
1 | Salmonella spp. |
2 | Corynebacterium kutscheri |
3 | Pasteurella pneumotropica |
4 | Klebsiella pneumoniae |
5 | Staphylococcus aureus |
6 | Pseudomonas aeruginosa |
7 | Ectoparasites |
8 | Ectromelia Virus |
9 | Mouse Hepatitis Virus (MHV) |
10 | Sendai Virus |
11 | Pneumonia Virus of Mice (PVM) |
12 | Reovirus 3 (Reo 3) |
13 | Minute Virus of Mice |
14 | Clostridium piliforme |
15 | Lymphocytic Choriomeningitis Virus |
16 | Hantaan Virus |
17 | Mouse Encephalomyelitis Virus(GDVII)/Rat Theilovirus (RTV) |
18 | Mouse Adenovirus I and II (FL and K88) |
19 | Polyoma Virus |
20 | Streptococcus pneumoniae |
21 | Encephalitozoon cuniculi |
22 | Beta hemolytic Streptococcus (non-Group D) |
23 | Mycoplasma spp |
24 | Mycoplasma Pulmonis |
25 | Enteric Helminths |
26 | Endoparasites |
27 | Ciliates |
28 | Toxoplasma |
29 | Bordetella bronchiseptica |
30 | Pasteurella multocida |
31 | Klebsiella oxytoca |
32 | Citrobacter rodentium |
33 | Proteus spp. |
34 | Mouse Parvovirus (MPV) |
35 | Murine Norovirus (MNV) |
36 | Mouse Rotavirus (EDIM) |
37 | K Virus |
38 | Mouse Cytomegalovirus (MCMV) |
39 | Cilia Associated Respiratory Bacillus |
40 | Thymic Virus |
41 | Lactate Dehydrogenase Elevating Virus |
42 | Helicobacter spp. |
43 | Streptococcus B-hemolytic (Groups B) |
44 | Streptococcus B-hemolytic (Groups G) |
45 | NS-1 |
Quality Control Statements of Genetic Background of Animals
Genotype Identification
Stage 1: Full Inspection
From generation F1 to generation F3, the first two embryos of each generation are analyzed consecutively, and 100% of modified targets are analyzed;
Stage 2: 20% Sampling Inspection
For generation F4, the first two embryos of each generation are analyzed consecutively, and 20% of modified targets are analyzed;
For offspring produced by IVF based on homozygote, sampling inspection is also performed at a sampling ratio of 20%
Stage 3: 5% Sampling Inspection
For generation F5, the first two embryos of each generation are analyzed consecutively, and 5% of modified targets are analyzed;
Stage 4: Quarterly Sampling Inspection
From generation F6 to generation F7, 5% of modified targets are analyzed every quarter;
Stage 5: Annual Sampling Inspection
From generation F8 on, for each generation with a population size of greater than 1000, 16 animals are analyzed every month;
For each generation with a population size below 1000, quarterly sampling inspection at a sampling ratio of 5% is maintained;
A comprehensive genetic background test is carried out each year (to determine the number of corresponding sites depending on characteristics of the investigated mice)
Note: for each mouse, its tail is cut off prior to weaning, and for each back-up mouse left for species conservation, its tail is cut off again prior to mating. (i.e., when back-up parental mice weaned at the age of 3 weeks have been fed for 6 weeks, one collective tail cutting is arranged for genotype verification);
Normal testing will not be resumed until stage 4