Quality Assurance (QA) & Quality Control (QC)

Animal Microbiology

Quarterly Testing Sheet for B-NDG Immunodeficienct Mice
ECT Mycoplasma spp. Group G beta-hemolytic Streptococcus
MHV Corynebacterium kutscheri In-vitro parasites (arthropods, such as fleas, mites, and lice)
SV Pasteurella pneumotropica Enteric Helminths
PVM Klebsiella pneumoniae Flagellates
REO-3 Staphylococcus aureus Ciliates
MMV/MVM Pseudomonas aeruginosa Toxoplasma gondii
LCMV Salmonella group Pneumocystis carinii
HV Yersinia pseudotuberculosis* Encephalitozoon cuniculi
TMEV Yersinia enterocolitica* Amoeba limicola
MAV/MAD1, 2 Streptobacillus moniliformis* Proteus mirabilis
POLY Pathogenic dermal fungi*
MPV Escherichia coli O115 a,C,K(B)*
EDIM Streptococcus pneumoniae*
MNV Beta-hemolytic streptococcus*
MCMV Klebsiella oxytoca*
KVIrus Corynebacterium bovis
Parvovirus NS-1 Helicobacter
Lactate dehydrogenase-elevating virus CARB
Clostridium piliforme Group B beta-hemolytic Streptococcus

Quarterly Testing Sheet for Immune Checkpoint Humanized Mice
No. Test Item
1 Salmonella spp.
2 Corynebacterium kutscheri
3 Pasteurella pneumotropica
4 Klebsiella pneumoniae
5 Staphylococcus aureus
6 Pseudomonas aeruginosa
7 Ectoparasites
8 Ectromelia Virus
9 Mouse Hepatitis Virus (MHV)
10 Sendai Virus
11 Pneumonia Virus of Mice (PVM)
12 Reovirus 3 (Reo 3)
13 Minute Virus of Mice
14 Clostridium piliforme
15 Lymphocytic Choriomeningitis Virus
16 Hantaan Virus
17 Mouse Encephalomyelitis Virus(GDVII)/Rat Theilovirus (RTV)
18 Mouse Adenovirus I and II (FL and K88)
19 Polyoma Virus
20 Streptococcus pneumoniae
21 Encephalitozoon cuniculi
22 Beta hemolytic Streptococcus (non-Group D)
23 Mycoplasma spp
24 Mycoplasma Pulmonis
25 Enteric Helminths
26 Endoparasites
27 Ciliates
28 Toxoplasma
29 Bordetella bronchiseptica
30 Pasteurella multocida
31 Klebsiella oxytoca
32 Citrobacter rodentium
33 Proteus spp.
34 Mouse Parvovirus (MPV)
35 Murine Norovirus (MNV)
36 Mouse Rotavirus (EDIM)
37 K Virus
38 Mouse Cytomegalovirus (MCMV)
39 Cilia Associated Respiratory Bacillus
40 Thymic Virus
41 Lactate Dehydrogenase Elevating Virus
42 Helicobacter spp.
43 Streptococcus B-hemolytic (Groups B)
44 Streptococcus B-hemolytic (Groups G)
45 NS-1



Quality Control Statements of Genetic Background of Animals

Genotype Identification

Stage 1: Full Inspection

From generation F1 to generation F3, the first two embryos of each generation are analyzed consecutively, and 100% of modified targets are analyzed;

Stage 2: 20% Sampling Inspection

For generation F4, the first two embryos of each generation are analyzed consecutively, and 20% of modified targets are analyzed;

For offspring produced by IVF based on homozygote, sampling inspection is also performed at a sampling ratio of 20%

Stage 3: 5% Sampling Inspection

For generation F5, the first two embryos of each generation are analyzed consecutively, and 5% of modified targets are analyzed;

Stage 4: Quarterly Sampling Inspection

From generation F6 to generation F7, 5% of modified targets are analyzed every quarter;

Stage 5: Annual Sampling Inspection

From generation F8 on, for each generation with a population size of greater than 1000, 16 animals are analyzed every month;

For each generation with a population size below 1000, quarterly sampling inspection at a sampling ratio of 5% is maintained;

A comprehensive genetic background test is carried out each year (to determine the number of corresponding sites depending on characteristics of the investigated mice)


Note: for each mouse, its tail is cut off prior to weaning, and for each back-up mouse left for species conservation, its tail is cut off again prior to mating. (i.e., when back-up parental mice weaned at the age of 3 weeks have been fed for 6 weeks, one collective tail cutting is arranged for genotype verification);

Normal testing will not be resumed until stage 4

Back to top