Description
Description:
Biocytogen designed a targeted mutation mouse named B-Cd155-EGFP KI mice which Cd155 gene was replaced with the EGFP gene.
Application:
Validation of the function of Cd155 in the development of tumor disease.
Protein expression analysis in B cell
Protein expression analysis in T cell
Protein expression analysis in NK cell
Protein expression analysis in NK cell
Protein expression analysis in macrophages
Protein expression analysis in monocytes
Protein expression analysis in granulocytes
Tageting strategy
Gene targeting strategy for B-Cd155-EGFP KI mice. The EGFP gene encoding green fluorescent protein was inserted after the 5 'UTR sequences of mouse Cd155 gene to replace it in B-Cd155-EGFP mice.
Protein expression analysis in B cell
Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Spleen，bone marrow，lymph node and blood were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was not detectable in B cells of wild-type mice and homozygous B-Cd155-EGFP KI mice. EGFP was detectable in B cells of homozygous B-Cd155-EGFP KI mice.
Protein expression analysis in T cell
Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Spleen, lymph node, thymus and blood were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was detectable in T cells of wild-type mice, but not detectable in T cells of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in T cells of homozygous B-Cd155-EGFP KI mice.
Protein expression analysis in NK cell
Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Spleen, thymus, bone marrow and lymph node were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was detectable in NK cells of wild-type mice, but not detectable in NK cells of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in NK cells of homozygous B-Cd155-EGFP KI mice.
Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Blood was collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was not detectable in NK cells of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in NK cells of homozygous B-Cd155-EGFP KI mice.
Protein expression analysis in macrophages
Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Lavage solution, bone marrow, spleen and blood were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was detectable in macrophages of wild-type mice, but not detectable in macrophages of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in macrophages of homozygous B-Cd155-EGFP KI mice.
Protein expression analysis in monocytes
Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Lavage solution, bone marrow, spleen and blood were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was detectable in monocytes of wild-type mice, but not detectable in monocytes of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in monocytes of homozygous B-Cd155-EGFP KI mice.
Protein expression analysis in granulocytes
Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Spleen, blood and bone marrow were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was detectable in granulocytes of wild-type mice, but not detectable in granulocytes of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in granulocytes of homozygous B-Cd155-EGFP KI mice.
