Description
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene editing strategy
A target vector was
designed to insert iCre-WPRE-PA cassette into mouse endogenous genes 5 'UTR
downstream of GFAP. The targeting vector was electroporated into
C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were
injected into blastocysts and resulted chimeric mice. These mice were
maintained on a C57BL/6 background.
Phenotype analysis
In situ hybridization in hippocampal CA1 area to confirm µR selective
deletion from µRGFAP-/- mice with probes of µR mRNA (red),
GAD2/ GFAP/vGlut1 mRNA (green), and DAPI nucleus staining (blue).
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-Gfap-iCre mice] (Cat# 110144) was purchased from Biocytogen.
