B-Gfap-iCre mice

C57BL/6-Gfaptm1(icre)Bcgen/Bcgen • 110144

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B-Gfap-iCre mice

Catalog Number
110144
Strain Name
C57BL/6-Gfaptm1(icre)Bcgen/Bcgen
Strain Background
C57BL/6
NCBI gene ID
14580 (Mouse)
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  • Description
  • Targeting strategy
  • Phenotypic analysis

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      Description


      This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

      Gene editing strategy
      A target vector was designed to insert iCre-WPRE-PA cassette into mouse endogenous genes 5 'UTR downstream of GFAP. The targeting vector was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulted chimeric mice. These mice were maintained on a C57BL/6 background.
      Phenotype analysis

      In situ hybridization in hippocampal CA1 area to confirm µR selective

      deletion from µRGFAP-/- mice with probes of µR mRNA (red),

      GAD2/ GFAP/vGlut1 mRNA (green), and DAPI nucleus staining (blue).

      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-Gfap-iCre mice] (Cat# 110144) was purchased from Biocytogen.