• Relaxin R1 (Relaxin Receptor 1), also known as RXFP1 (Relaxin Family Peptide Receptor 1) or LGR7 (Leucine‑rich G‑protein‑coupled Receptor 7) is a member of family C of the LGRs, and is one of four receptors for Relaxin family proteins. Relaxin R1 shows highest affinity for human Relaxins 1, 2 and 3, while RXFP2 binds Relaxin 2 and the related INSL3, and RXFP3 primarily binds Relaxin 3.
• Engagement of Relaxin R1 by Relaxin (mainly Relaxin 2 in humans) supports female reproduction by promoting uterine angiogenesis, ovarian follicle ripening, and endometrial, cervical and nipple development. In male reproduction, Relaxin R1 acts in the prostate to enhance sperm motility. It reduces fibrosis in the heart, skin, lungs, liver, kidney, and reproductive tissues by combating aberrant collagen buildup. In the vasculature, it mediates vasodilation and decreases blood pressure.
• A human CDS that encodes full long human protein, followed by human 3’UTR is inserted in mouse Rxfp1 gene. The Human RXFP1 protein expression will be driven by mouse endogenous promoter, while mouse Rxfp1 gene transcription and translation will be disrupted.
• Mouse Rxfp1 mRNA was detectable in the wild-type C57BL/6 mice. Human RXFP1 mRNA was detectable in homozygous B-hRXFP1 mice but not in wild-type mice. High expression in testis, Uterus, Ovaries and Brain.
• Human and mouse RXFP1 were detectable in both wild-type and homozygous B-hRXFP1 mice by western blot,  anti-RXFP1 antibody was cross-reactive between human and mouse.
• Human and mouse RXFP1 were expressed in heart, liver, lung, Kidney, Brain, Stomach , Colon, Uterus, Ovaries and testis

B-hRXFP1 mice

• A human CDS that encodes full long human protein, followed by human 3’UTR is inserted in mouse exon17 (IRES2 as linker) to replace the Exon17~18 of mouse Rxfp1 gene.
• The Human RXFP1 protein expression will be driven by mouse endogenous promoter, while mouse Rxfp1 gene transcription and translation will be disrupted.

• Human RXFP1 mRNA was detectable only in homozygous B-hRXFP1 mice but not in wild-type mice.

Strain specific analysis of RXFP1 mRNA expression in wild-type C57BL/6 mice and B-hRXFP1 mice by RT-PCR. Brain(A), lung (B)and Heart(C) RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hRXFP1 mice(H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human RXFP1 primers. Mouse Rxfp1 mRNA were detectable in wild-type C57BL/6 mice. Human RXFP1 mRNA was detectable only in homozygous B-hRXFP1 mice but not in wild-type mice.

• Human RXFP1 mRNA was high expression in testis, Uterus, Ovaries and Brain.

Species specific analysis of human RXFP1 gene expression in homozygous B-hRXFP1 mice by RT-qPCR. Various tissue RNA were collected from homozygous B-hRXFP1 mice (H/H) (female, n=3, 7-week-old; male, n=3, 7-week-old). A. The mRNA expression of human RXFP1 in homozygous female B-hRXFP1 mice. B. The mRNA expression of human RXFP1 in homozygous male B-hRXFP1 mice.

• Human and mouse RXFP1 were expressed in heart, liver, lung, Kidney, Brain, Stomach , Colon, Uterus, Ovaries and testis.

Western blot analysis of RXFP1 protein expression in wild-type C57BL/6JNifdc mice and homozygous B-hRXFP1 mice. Various tissue lysates were collected from female(A) and male(B),wild-type C57BL/6JNifdc mice (+/+) (1♂1♀, 7-week-old) and homozygous B-hRXFP1 mice (H/H) (1♂1♀, 7-week-old), and then analyzed by western blot with recombinantly Anti-LGR7 antibody (GeneTex, GTX108186, the antibody was cross-reactive between human and mouse). 40 μg total proteins were loaded for western blotting analysis.

• Most indicators showed no significant differences compared with wild-type mice. However, three parameters including RBC, HGB and HCT were slightly decreased in female mice compared with wild-type , suggesting that female animals may develop mild anemia.

Values are expressed as mean ± SD.

• Most indicators showed no significant differences compared with wild-type mice.

Values are expressed as mean ± SD.

• No abnormalities were observed.

Organs of female B-hRXFP1 mice  (8-week-old, n = 10).

• No abnormalities were observed.

Organs of male B-hRXFP1 mice  (8-week-old, n = 10).

• No abnormalities were observed.

Average weights of major organs in B-hRXFP1 mice. Values are expressed as mean ± SD.

• No obvious abnormalities were observed in any organs examined (heart, liver, spleen, lung, kidney, brain, stomach, small intestine, Large intestine, ovary and testis).

Histopathological analysis of organs in B-hRXFP1 mice. Major organs from B-hRXFP1 mice were collected at 8 weeks of age and analyzed by H&E staining (male, n = 10; female, n = 10;  200x).