Targeting strategy
Gene targeting strategy for B-hCD70 mice.
The exons 1-3 of mouse Cd70 gene that encode the extracellular domain were replaced by human CD70 exons 1-3 in B-hCD70 mice.
mRNA expression analysis
Strain specific analysis of CD70 gene expression in wild type (WT) mice and B-hCD70 mice by RT-PCR. Mouse CD70 mRNA was detectable only in thymocytes of WT mice (+/+) and B-hCD70 mice (H/+). Human CD70 mRNA was detectable only in homozygous B-hCD70 mice (H/+) but not in WT mice (+/+).
Protein expression analysis-Dendritic cells
Strain specific CD70 expression analysis in homozygous B-hCD70 mice by flow cytometry. Bone marrow cells were collected from wild type (WT) mice (+/+) and homozygous B-hCD70 mice (H/H) stimulated with LPS in vivo, and analyzed by flow cytometry with species-specific anti-CD70 antibody. Mouse CD70 was detectable in WT mice (+/+). Human CD70 was exclusively detectable in homozygous B-hCD70 mice (H/H) but not in WT mice (+/+).
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hCD70 mice] (Cat# 110770) was purchased from Biocytogen.
