Basic Information
A construct including the iCre–WPRE-pA cassette was inserted upstream of the ATG start codon of the Fabp4 gene. Meantime, the expression of Fabp4 gene was blocked. These mice were maintained on a C57BL/6N background.
Description
Homozygous null mice are fertile and display impaired T cell and macrophage cytokine production.
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Targeting Strategy
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Phenotype Analysis
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Scheme for generating RhebAD KO mice. The RhebFL/FL mice were crossed with Fabp4-Cre mice (Fabp4-Cre mice were purchased from Biocytogen Co.Ltd., Beijing, China) to obtain RhebAD KO mice. Rheb was not detected in the interscapular BAT, inguinal and epididymal WAT of RhebAD KO mice. Compared to control mice, the interscapular BAT pads of RhebAD KO mice were significantly lighter and smaller, while no significant differences were observed in the inguinal and epididymal WAT.
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9.1Numbering rules of toe clipping for identification of mouse/rat model
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