Basic Information
Strain Name
C57BL/6N-Fabp4tm1(icre)Bcgen/Bcgen
Stock Number
110152
Common Name
B-Fabp4-iCre mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Background
C57BL/6
Appearance
Black
Development
A construct including the iCre–WPRE-pA cassette was inserted upstream of the ATG start codon of the Fabp4 gene. Meantime, the expression of Fabp4 gene was blocked. These mice were maintained on a C57BL/6N background.
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Phenotype Analysis
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Scheme for generating RhebAD KO mice. The RhebFL/FL mice were crossed with Fabp4-Cre mice (Fabp4-Cre mice were purchased from Biocytogen Co.Ltd., Beijing, China) to obtain RhebAD KO mice. Rheb was not detected in the interscapular BAT, inguinal and epididymal WAT of RhebAD KO mice. Compared to control mice, the interscapular BAT pads of RhebAD KO mice were significantly lighter and smaller, while no significant differences were observed in the inguinal and epididymal WAT.
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Instructions of genomic DNA extraction
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chematic representation of the toe clipping.
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Gene editing strategy
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References
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- Inouye KE, Prentice KJ, Lee A, et al. Endothelial-derived FABP4 constitutes the majority of basal circulating hormone and regulates lipolysis-driven insulin secretion. JCI Insight. 2023;8(14):e164642. Published 2023 Jul 24. doi:10.1172/jci.insight.164642
- Wang T, Zhao C, Zhang J, et al. Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9. iScience. 2024;27(2):108857. Published 2024 Jan 11. doi:10.1016/j.isci.2024.108857.
- Zhou M, Bao Y, Li H, et al. Deficiency of adipocyte fatty-acid-binding protein alleviates myocardial ischaemia/reperfusion injury and diabetes-induced cardiac dysfunction. Clin Sci (Lond). 2015;129(7):547-559. doi:10.1042/CS20150073
