Basic Information
An F2A-iCre sequence cassette was placed between the coding sequence of exon 2 and 3’UTR of the Sp7 gene in C57BL/6 ES cells. This strain was maintained on a C57BL/6 genetic background.
This Sp7iCre model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of bone development, osteoblast lineage, and Hedgehog/Wnt signaling.
Description
The Sp7 gene is a C2H2-type zinc finger transcription factor of the SP gene family and putative master regulator of bone cell differentiation. In this strain, Cre recombinase expression is under the control of Sp7 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in Sp7 expressing cells.
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Publications using B-Sp7-iCre mice
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Liu, X., Jiang, L., Zhang, W., Zhang, J., Luan, X., Zhan, Y., Wang, T., Da, J., Liu, L., Zhang, S., Guo, Y., Zhang, K., Wang, Z., Miao, N., Xie, X., Liu, P., Li, Y., Jin, H., & Zhang, B. (2023). Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis. Journal of translational medicine, 21(1), 417. https://doi.org/10.1186/s12967-023-04275-4
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Phenotype Analysis
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Fig 4. qPCR, Western blot, and Immunofluorescence analyses showed that Fam20c knockout resulted in a decreased expression of Calpastatin in OB Fam20cKO cells (Fig. 4A, B, E), whereas accompanied by a decreased phosphorylated expression level of Calpastatin (Fig. 4C). These were consistent with the results of proteomics and phosphoproteomics. Furthermore, Fam20cdeficient osteoblasts displayed a diminished expression of Calpain 1 and Calpain 2 (Fig. 4A, B, E) and attenuation of Calpain activity (Fig. 4D).
(source: Liu et al. Journal of Translational Medicine (2023) 21:417)
