Basic Information

Strain Name
C57BL/6-Sp7tm1(icre)Bcgen/Bcgen
Stock Number
110131
Common Name
B-Sp7-F2A-iCre mice
Gene symbol and name
Sp7(transcription factor 7), Osx
Background
C57BL/6
Appearance
Black
Development

An F2A-iCre sequence cassette was placed between the coding sequence of exon 2 and 3’UTR of the Sp7 gene in C57BL/6 ES cells. This strain was maintained on a C57BL/6 genetic background.

NCBI Gene ID
Application
Function research of genes

This Sp7iCre model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of bone development, osteoblast lineage, and Hedgehog/Wnt signaling.

Description

The Sp7 gene is a C2H2-type zinc finger transcription factor of the SP gene family and putative master regulator of bone cell differentiation. In this strain, Cre recombinase expression is under the control of Sp7 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in Sp7 expressing cells.

Publications using B-Sp7-iCre mice

Liu, X., Jiang, L., Zhang, W., Zhang, J., Luan, X., Zhan, Y., Wang, T., Da, J., Liu, L., Zhang, S., Guo, Y., Zhang, K., Wang, Z., Miao, N., Xie, X., Liu, P., Li, Y., Jin, H., & Zhang, B. (2023). Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis. Journal of translational medicine, 21(1), 417.

Songlin Peng, Chungeng Liu, Naibo Feng et al. Foxk1 promotes bone formation through inducing aerobic glycolysis, 28 May 2024, PREPRINT (Version 1) available at Research Square

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis

Knocking out FAM20C in pre-osteoblasts leads to up-regulation of osteoclast differentiation to affect long bone development

Histone demethylase KDM7A regulates bone homeostasis through balancing osteoblast and osteoclast differentiation

Ptip safeguards the epigenetic control of skeletal stem cell quiescence and potency in skeletogenesis

Phenotype Analysis

Fig 4. qPCR, Western blot, and Immunofluorescence analyses showed that Fam20c knockout resulted in a decreased expression of Calpastatin in OB Fam20cKO cells (Fig. 4A, B, E), whereas accompanied by a decreased phosphorylated expression level of Calpastatin (Fig. 4C). These were consistent with the results of proteomics and phosphoproteomics. Furthermore, Fam20cdeficient osteoblasts displayed a diminished expression of Calpain 1 and Calpain 2 (Fig. 4A, B, E) and attenuation of Calpain activity (Fig. 4D).

(source: Liu et al. Journal of Translational Medicine (2023) 21:417)

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