B-Cd11b-EGFP-DTR-Luc mice

Basic Information

Strain name
C57BL/6JNifdc-Itgamtm1(EGFP-DTR-Luc)Bcgen/Bcgen
Common name
B-Cd11b-EGFP-DTR-Luc mice
Background
C57BL/6JNifdc
Catalog number
112825
Aliases
NA
NCBI gene ID
NA

Targeting strategy

Gene targeting strategy for B-Cd11b-EGFP-DTR-Luc mice. 

CD11b co-express EGFP,  the DTR for depletion of CD11b+ cells, and the luciferase for in vivo bioluminescent imaging (BLI) of CD11b+ cells. EGFP-DTR-Luc expression is evident in CD11b+ cells.  

Protein expression analysis

Strain specific EGFP expression analysis in heterozygous B-Cd11b-EGFP-DTR-Luc mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and heterozygous B-Cd11b-EGFP-DTR-Luc mice (Mut/+), and analyzed by flow cytometry. EGFP was not detectable in B cells, T cells, while EGFP was detectable in NK cells and  CD11b+ cells of heterozygous mice.

Strain specific EGFP expression analysis in heterozygous B-Cd11b-EGFP-DTR-Luc mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and heterozygous B-Cd11b-EGFP-DTR-Luc mice (Mut/+), and analyzed by flow cytometry. EGFP was detectable in DCs, neutrophils, monocytes and macrophages of heterozygous mice.

Frequency of leukocyte subpopulations in spleen

Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice (+/+) and heterozygous B-Cd11b-EGFP-DTR-Luc mice (Mut/+) (n=3, 9-week-old) injected with PBS or DT (50 ng/g body weight) for three consecutive days. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. Values are expressed as mean ± SEM.

CD11b+ cells depletion analysis

Frequencies of CD11b+ EGFP+ cells and EGFP+ cells in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice (+/+) and heterozygous B-Cd11b-EGFP-DTR-Luc mice (Mut/+) (n=3, 9-week-old) injected with PBS or DT (50 ng/g body weight) for three consecutive days. Flow cytometry analysis of the splenocytes was performed to assess the frequencies of CD11b+ EGFP+ cells and EGFP+ cells in total CD45+ cells. The frequencies of CD11b+ EGFP+ cells (A) and EGFP+ cells (B) were decreased in heterozygous mice injected with DT.

Frequencies of B cells, T cells, NK cells and DCs in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice (+/+) and heterozygous B-Cd11b-EGFP-DTR-Luc mice (Mut/+) (n=3, 9-week-old) injected with PBS or DT (50 ng/g body weight) for three consecutive days. Flow cytometry analysis of the splenocytes was performed to assess the frequencies of B cells, T cells, NK cells and DCs in total CD45+ cells. The frequencies of NK cells and DCs (B) were decreased in heterozygous mice injected with DT, while the frequencies of B cells and T cells (A) were not changed.

Frequencies of neutrophils, monocytes and macrophages in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice (+/+) and heterozygous B-Cd11b-EGFP-DTR-Luc mice (Mut/+) (n=3, 9-week-old) injected with PBS or DT (50 ng/g body weight) for three consecutive days. Flow cytometry analysis of the splenocytes was performed to assess the frequencies of neutrophils, monocytes and macrophages in total CD45+ cells. The frequencies of neutrophils (A), monocytes (B) and macrophages (B) were decreased in heterozygous mice injected with DT.

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