Basic Information

Strain Name
Common Name
B-hGDF15 mice
Catalog Number
Related Gene
growth differentiation factor 15, GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB

Gene targeting strategy

Gene targeting strategy for B-hGDF15 mice. The exons 1-2 of mouse Gdf15 gene that encode the full-length protein were replaced by human GDF15 exons 1-2 in B-hGDF15 mice.

mRNA expression analysis

Species-specific GDF15 gene expression analysis in wild-type and humanized B-hGDF15 mice by RT-PCR. Murine Gdf15 mRNA was detected in kidney tissue isolated from wild-type (+/+) mice, while human GDF15 mRNA was detected in B-hGDF15 mice.

Protein expression analysis

Species-specific GDF15 expression analysis in homozygous B-hGDF15 mice. Serum was collected from wild-type (+/+) and B-hGDF15 (H/H) mice and analyzed using a species-specific GDF15 ELISA kit. Mouse GDF15 expression was detectable in wild-type mice, while human GDF15 expression was exclusively detectable in B-hGDF15 (H/H) mice compared to wild-type.


mRNA expression:

Mouse Gdf15 mRNA was detectable in kidney of wild-type (+/+). Human GDF15 mRNA was detectable only in H/H but not in +/+ mice. 

Protein  expression:

Mouse GDF15 was exclusively detectable in wild-type mice and human GDF15 was exclusively detectable in homozygous B-hGDF15 mice.

Back to top