Gene targeting strategy for B-hGDF15 mice.
The exons 1-2 of mouse Gdf15 gene that encode the full-length protein were replaced by human GDF15 exons 1-2 in B-hGDF15 mice.
Protein expression analysis
Strain specific GDF15 expression analysis in heterozygous B-hGDF15 mice by ELISA.
Serum was collected from C57BL/6 wild-type mice (+/+) and heterozygous B-hGDF15 mice (H/+) analyzed by ELISA with species-specific GDF15 ELISA kit. Mouse GDF15 were both detectable in wild-type mice and heterozygous B-hGDF15 mice. Human GDF15 was exclusively detectable in heterozygous B-hGDF15 mice but not in wild-type mice. ND: not detected.