Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hGDF15 mice. The exons 1-2 of mouse Gdf15 gene that encode the full-length protein were replaced by human GDF15 exons 1-2 in B-hGDF15 mice.
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mRNA expression analysis
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Species-specific GDF15 gene expression analysis in wild-type and humanized B-hGDF15 mice by RT-PCR. Murine Gdf15 mRNA was detected in kidney tissue isolated from wild-type (+/+) mice, while human GDF15 mRNA was detected in B-hGDF15 mice.
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Protein expression analysis
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Species-specific GDF15 expression analysis in homozygous B-hGDF15 mice. Serum was collected from wild-type (+/+) and B-hGDF15 (H/H) mice and analyzed using a species-specific GDF15 ELISA kit. Mouse GDF15 expression was detectable in wild-type mice, while human GDF15 expression was exclusively detectable in B-hGDF15 (H/H) mice compared to wild-type.
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Summary
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mRNA expression：
Mouse Gdf15 mRNA was detectable in kidney of wild-type (+/+). Human GDF15 mRNA was detectable only in H/H but not in +/+ mice.
Protein expression：
Mouse GDF15 was exclusively detectable in wild-type mice and human GDF15 was exclusively detectable in homozygous B-hGDF15 mice.
